We narrowed to 9,789 results for: Coli
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Plasmid#174719PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSunS 1-335
Plasmid#171673PurposeSunS 1-335 expression in E. coliDepositorInsertGlycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
Tags6x HISExpressionBacterialPromoterT7Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR300.3
Plasmid#125079PurposeExpression of uhpA under PLtetO-1, output Promoter PuhpT99DepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2-R232Q
Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
ExpressionBacterialPromotertacAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA-T679A
Plasmid#118936PurposeUsed to express the T679A mutant form of the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
ExpressionBacterialAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only