Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,636 results for: pcr

Clear keywords
Showing: 1521 - 1540 of 4636 results

  1. pAAH4

    Plasmid
    #41828
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41828-plasmid-map-sequence-id-69199

    Enlarge

  3. pBP-lacZ

    Plasmid
    #72948
    Purpose
    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - Bioparts
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesiā€¦
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72948-plasmid-map-sequence-id-133793

    Enlarge

  4. pJW2072

    Plasmid
    #154334
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154334-plasmid-map-sequence-id-318659

    Enlarge

  6. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

    Enlarge

  8. pJL357

    Plasmid
    #243711
    Purpose
    A piggyBac vector containing the COX7A2 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of COX7A2 (COX7A2 Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243711-plasmid-map-sequence-id-489280

    Enlarge

  17. pSG424 ELKC(S383A)

    Plasmid
    #89334
    Purpose
    Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.
    Insert
    ELK1 (ELK1 Human)
    Tags
    Gal 4
    Expression
    Mammalian
    Mutation
    encodes aa 307-428, changed serine 383 to alanineā€¦
    Available Since
    April 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    89334-plasmid-map-sequence-id-176433

    Enlarge

Showing: 1521 - 1540 of 4636 results