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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-eGFP

Plasmid

#80344

Purpose

Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
eGFP

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKA-207I10

Plasmid

#79845

Purpose

Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000563266)

Plasmid

#80036

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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