-
Plasmid#227264PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(1)
Plasmid#62893PurposeTrojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg
Plasmid#159927PurposeGene targetingDepositorInsertERT2CreERT2
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFvL118
Plasmid#64752PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW081
Plasmid#64755PurposeRecombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKV016
Plasmid#64767PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHPK1212
Plasmid#8834DepositorInserttobacco vein mottling virus protease, catalytic domain
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dNmeCas9
Plasmid#120077PurposedNmeCas9 with no sgRNA cassette lenti constructDepositorInsertdNmeCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
3087 pBS Bcl-2-Ig
Plasmid#8809DepositorInsertBcl-2-Ig (BCL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-PGK-Blast
Plasmid#227265PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Zeo
Plasmid#227261PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL390
Plasmid#219500PurposeIntegrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpressionDepositorInsertIntegrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
UseTagsSV40-NLSExpressionYeastMutationPromoterGAL1-GAL10Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
S111
Plasmid#174549PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)DepositorInsertBclxL and designed protein LD3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
S112
Plasmid#174550PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)DepositorInsertBcl2 and designed protein LD3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW087
Plasmid#64756PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CW3SL-EGFP
Plasmid#61463PurposeAAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only