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Plasmid#163717PurposePlasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)DepositorInsertsgRNA expression cassette
UseSynthetic Biology; Moclo vector level 0 (type 234)TagsExpressionMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC0_4_VPR
Plasmid#163716PurposePlasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertVPR
UseSynthetic Biology; Moclo vector level 0 (type 4a)TagsExpressionMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174528PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertPylS
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CT-Tagging
Plasmid#165871PurposeTemplate for amplification of EGFP C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xP3
Plasmid#165779Purpose3xP3 eye driver enhancer element, requires promoter element for functionDepositorInsert3xP3
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CT-Tagging
Plasmid#165869PurposeTemplate for amplification of mcherry C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NT-Tagging
Plasmid#165868PurposeTemplate for amplification of EGFP N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV40L-TN
Plasmid#165827PurposeViral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRsDepositorInsertSV40L
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 CT
Plasmid#165811PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 partsDepositorInsertGGSx4 CT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-A1
Plasmid#165814PurposeAlpha1 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-A1
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-A2
Plasmid#165815PurposeAlpha2 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-A2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-O2
Plasmid#165816PurposeOmega2 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-O2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-O1
Plasmid#165817PurposeOmega1 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-O1
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLexA BD
Plasmid#165821PurposeLexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertLexA BD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFBD
Plasmid#165823PurposeQF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertQFBD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFAD
Plasmid#165824PurposeQF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertQFAD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 NT
Plasmid#165810PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 partsDepositorInsertGGSx4 NT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCP6-Dros
Plasmid#165794PurposeFusion grammar bacterial CP6 promoter, assembles with pHSP70B-TACTDepositorInsertCP6 Promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCP7-Dros
Plasmid#165796PurposeFusion grammar bacterial CP7 promoter, assembles with pHSP70B-TACTDepositorInsertCP7 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCS-TCCC
Plasmid#165774PurposeMultiple cloning site part for enhancer cloning, requires minimal promoter elementDepositorInsertMCS-TCCC
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdSCP
Plasmid#165776PurposeMinimal synthetic Drosophila core promoter, requires enhancer element for full expressionDepositorInsertdSCP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#2
Plasmid#163395PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#1
Plasmid#163394PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#3
Plasmid#163396PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB2
Plasmid#168472PurposeN-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split proteinDepositorInsertN-fragment of DiB2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-ble
Plasmid#165880PurposeBasic Phleomycin resistance cassette in Alpha1, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NT-Tagging
Plasmid#165870PurposeTemplate for amplification of mCherry N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISH-Olfr1508
Plasmid#136746PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr1508DepositorInsertOlfr1508 (Or4e1 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insertPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only