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Showing: 1541 - 1560 of 32215 results

  2. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Use
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

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  3. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  4. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  9. Epac-S-H30

    Plasmid
    #170337
    Purpose
    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Insert
    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170337-plasmid-map-sequence-id-344639

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  10. pICE-HA-Ku70-siR-Mut6E

    Plasmid
    #82330
    Purpose
    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10
    Insert
    Ku70 (XRCC6 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV-tet
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    82330-plasmid-map-sequence-id-155730

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  13. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid
    #165436
    Purpose
    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…
    Promoter
    CMV
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    165436-plasmid-map-sequence-id-325943

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  15. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  16. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid
    #194193
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194193-plasmid-map-sequence-id-381931

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  17. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Article
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Insert
    Protein S
    Use
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

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  18. pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

    Plasmid
    #169174
    Purpose
    piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    SOX17-P2A-Venus (SOX17 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTREtight (tet-inducible promoter)
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    169174-plasmid-map-sequence-id-348368

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  19. JA412

    Plasmid
    #49935
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD KL-1 (KLF4 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pGL3-Enhancer-ZC3H12D_PromoterMUT

    Plasmid
    #153068
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE40 binding sites. N…
    Promoter
    None
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    153068-plasmid-map-sequence-id-304889

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Showing: 1541 - 1560 of 32215 results