We narrowed to 8,420 results for: construct

1. pJK585

   Plasmid

   #72458

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes tryptophan-34 to phenylalanine, …

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTF257 (pFA6a-TEV-6xGly-His3MX)

   Plasmid

   #44068

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF258 (pFA6a-PSc-6xGly-His3MX)

   Plasmid

   #44062

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTF261 (pFA6a-TEV-6xGly-HphMX)

   Plasmid

   #44080

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTF262 (pFA6a-PSc-6xGly-HphMX)

   Plasmid

   #44074

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF259 (pFA6a-TEV-6xGly-KanMX)

   Plasmid

   #44092

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PosmY, PsulA, and grpE

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTorPE-GEM-GECO1

   Plasmid

   #32463

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Lab constructed

   Tags

   6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

   Expression

   Bacterial

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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