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Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC
Plasmid#115248PurposeFor mammalian expression of the human NODAL open reading frame with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 specR cassette
Plasmid#117120PurposeSpectinomycin resistance cassetteDepositorInsertSpectinomycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-TO-Hygromycin-mVenus-MAP
Plasmid#44099DepositorTypeEmpty backboneUseTagsmVenus-MAPExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-6xHis
Plasmid#87343PurposeTo express EGFP-6xHis in mammalian cellDepositorInsert6xHis
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG/ Inducible ALPHA MHC
Plasmid#55792Purposetransgene expression driven by inducible ALPHA MHC promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterInducible ALPHA MHCAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL0_4 [pIJ101 ORI]
Plasmid#198921PurposeLevel 0 partDepositorInsertpIJ101 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLU
Plasmid#64167Purposeharbours marker genes HIS3, LEU2 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-MCS
Plasmid#188970PurposeFor red light-repressed bacterial expression of gene of interestDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - mSRSF6
Plasmid#120420Purposeoverexpression of mouse Srsf6DepositorInsertSRSF6 (Srsf6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
vgCam-pRSETb
Plasmid#193362PurposeA FRET-based calcium probe using SumireDepositorInsertvgCam
UseE.coli expressionTagsHis X6 tag and Strep tagExpressionMutationPromoterT7Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM291rfp
Plasmid#59775PurposepCMrfp with TrfA mutation (291E to G)DepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-T7-WspR:D70E
Plasmid#79164PurposeExpresses constitutively active WspR D70E under a T7 promoterDepositorInsertWspR (wspR P. areuginosa)
UseTagsExpressionBacterialMutationD70EPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_22 [pSG5 ORI]
Plasmid#198935PurposeLevel 0 partDepositorInsertpSG5 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_5 [pAL5000 ORI]
Plasmid#198922PurposeLevel 0 partDepositorInsertpAL5000 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLM
Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-SdB-T
Plasmid#87307PurposeExpresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.DepositorInsertStabilized dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03R122K/R126K
Plasmid#28005DepositorInsertAT1.03R122K/R126K
UseTagsmVenus and mseCFPExpressionYeastMutationR122K, R126KPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-T7-Ct-WspR
Plasmid#79166PurposeDual expression of Ct biosensor and WspR WT under individual T7 promotersDepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODAL
Plasmid#115638PurposeFor targeted integration and inducible expression of human NODAL using doxycyclineDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk3b
Plasmid#208986PurposeRiboprobe generationDepositorInsertkcnk3b (kcnk3b Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL11-GCaMP6f bb100
Plasmid#158983PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.DepositorInsertGCaMP6f
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23100 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mClover/nat
Plasmid#120330PurposeBacterial mClover labelingDepositorInsertmClover
UseTagsD103N (see comment below)ExpressionBacterialMutationPromoter(None)Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_19 [panARS ORI]
Plasmid#198932PurposeLevel 0 partDepositorInsertpanARS ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC97
Plasmid#124352PurposePlasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 mini-intein
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-ESyn1
Plasmid#80804PurposehSyn1 promoter fused to CMVminDepositorInsertp5E-ESyn1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-Top-T
Plasmid#87309PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFCK(1.3)GFP-SynaptophysW
Plasmid#27235DepositorInsertEGFP
UseLentiviralTagsSynaptophys-GFPExpressionMutationPromoterAvailable sinceMarch 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-Bottom-T
Plasmid#87310PurposeExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Bottom" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
UseTagsNLS-3xHAExpressionInsectMutationPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-CFP
Plasmid#1812DepositorInsertGag (gag MuLV)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A3B
Plasmid#43852DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A4A
Plasmid#43853DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STX6
Plasmid#31581DepositorInsertsyntaxin 6 (STX6 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
ACE16-NLS
Plasmid#210727PurposeNIR light-activated intein-based gene expression system (C-part)DepositorInsertACE16-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq1-10M1-sspBmyc
Plasmid#27069DepositorInserttetR-PmyctetO-sspB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_NT
Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Tev-Avi-tag
Plasmid#117128PurposeC-terminal Tev-Avi tag TagDepositorInsertTev Avi-tag
UseGolden gate donor vector for gene targeting in ba…TagsTev Avi-TagExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-ScbR
Plasmid#49370DepositorInsertScbR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL0_13 [pIP501 ORI]
Plasmid#198927PurposeLevel 0 partDepositorInsertpIP501 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE myc-Raptor S722A/S792A
Plasmid#18117DepositorInsertRaptor (RPTOR Human)
UseRetroviralTagsMycExpressionMammalianMutationS722A, S792APromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6p1-HH1713
Plasmid#124994PurposeE. coli expression of recombinant H. hepaticus antigen HH_1713DepositorInsertHH_1713
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-BALF2
Plasmid#72629PurposeMammalian expression vector for Epstein-Barr virus single-strand DNA binding proteinDepositorInsertBALF2
UseTagsExpressionMammalianMutationS1028P and V1088APromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only