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We narrowed to 15,547 results for: pET

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Showing: 3901 - 3950 of 15547 results

  3. pETDuet-solo-NHis6-NocO-D282A

    Plasmid
    #240407
    Purpose
    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Asp…
    Promoter
    T7
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240407-plasmid-map-sequence-id-485005

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  6. Ec (gamma-only)-pET(11a)(ACH–)

    Plasmid
    #241265
    Purpose
    Over-express E. coli Pol III subunit gamma-only in E. coli
    Depositor
    Michael O'Donnell
    Insert
    dnaXZ (gamma-only)
    Expression
    Bacterial
    Mutation
    No expression of tau
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241265-plasmid-map-sequence-id-482108

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  12. pETDuet-solo-NHis6-NocO-H286A

    Plasmid
    #240409
    Purpose
    expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240409-plasmid-map-sequence-id-478125

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  13. pETDuet-solo-NHis6-NocO-H132A

    Plasmid
    #240404
    Purpose
    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240404-plasmid-map-sequence-id-478123

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  14. pETDuet-solo-NHis6-NocO-E129A

    Plasmid
    #240403
    Purpose
    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240403-plasmid-map-sequence-id-478122

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  15. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid
    #240402
    Purpose
    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240402-plasmid-map-sequence-id-478121

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  16. pETDuet-solo-NHis6-NocO-E252A

    Plasmid
    #240405
    Purpose
    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240405-plasmid-map-sequence-id-478119

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  17. pETDuet-solo-NHis6-NocO-E283A

    Plasmid
    #240408
    Purpose
    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240408-plasmid-map-sequence-id-478118

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  18. pETDuet-solo-NHis6-NocO-H279A

    Plasmid
    #240406
    Purpose
    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240406-plasmid-map-sequence-id-478117

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  19. pETDuet-solo-NHis6-NocO-E94A

    Plasmid
    #240401
    Purpose
    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240401-plasmid-map-sequence-id-478116

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  20. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

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  21. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  32. pET/His-GST-Rab1a(Q70L)

    Plasmid
    #232911
    Purpose
    E. coli Expression
    Depositor
    James Hurley
    Insert
    RAB1A (RAB1A Human)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Q70L
    Available Since
    March 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232911-plasmid-map-sequence-id-482325

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  34. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid
    #225141
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 160N, mCherry
    Tags
    Ag43 protein fused to silaffin-R5 and mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225141-plasmid-map-sequence-id-460500

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  35. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid
    #229220
    Purpose
    For expression of T. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229220-plasmid-map-sequence-id-460016

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  37. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid
    #229221
    Purpose
    For expression of T. elongatus KaiB protein
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229221-plasmid-map-sequence-id-457227

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  38. pET28b-SUMO-SeKaiB-K25C-FLAG

    Plasmid
    #229225
    Purpose
    For expression of S. elongatus KaiB protein mutant
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB S. elongatus)
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Mutation
    K25C
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229225-plasmid-map-sequence-id-457071

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  44. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169134-plasmid-map-sequence-id-380322

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  47. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169133-plasmid-map-sequence-id-361153

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Showing: 3901 - 3950 of 15547 results