We narrowed to 15,323 results for: pET
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Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
Tags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
Tags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgPet-1-hSyn-mCherry-KASH
Plasmid#223227PurposeguideRNA targeting the mouse Pet-1 (Fev)DepositorInsertFev (Fev Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsmCherryExpressionMammalianPromoterU6Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b Salmonella NAT_6H C-terminal
Plasmid#223436PurposeSalmonella NAT expression vector with C-terminal His6 tagDepositorInsertSalmonella NAT
TagsHis6Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Cdc42/13C-FKBP-MBP
Plasmid#214291PurposeExpress split-Cdc42 fragment fused with FKBP and MBPDepositorInsertSplit-Cdc42 fragment fused with FKBP (CDC42 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Rac1/13C-FKBP-MBP
Plasmid#214295PurposeExpress split-Rac1 fragment fused with FKBP and MBPDepositorInsertSplit-Rac1 fragment fused with FKBP (RAC1 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
Tags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-effector-ncRNA-rrt-His8
Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
TagsHis8PromoterT7Available SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
Tags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF3
Plasmid#218194PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3DepositorInsertEndoF3
TagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 41-329PromoterT7Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-EmEndoF1
Plasmid#218195PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1DepositorInsertEndoF1
TagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 51-339PromoterT7Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
TagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
TagsSUMOExpressionBacterialMutationY96C, C139A, C141SAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_mouse-Pro-IL-18_humanized-triple-mutant
Plasmid#214339PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Mouse)
TagsHis-TEVExpressionBacterialMutationENGD29DDEN_N36Y_H41EPromoterT7Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
Tags6xHis-CUbo(K63R)ExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
Tags6xHis-CUbo(K48R)ExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-RPA4/His14 (MSW#949)
Plasmid#208075PurposeExpression of human Replication Protein A 4 and His-tagged RPA3 in E.coliDepositorInsertRPA4, RPA14(His-tagged)
Tags10xHis tag on RPA14ExpressionBacterialAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaR-SpCas9-NLS-6xHis
Plasmid#207387PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Blackjack SpCas9-NLS-6xHis
Plasmid#207375PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertBlackjack SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationamino acids 1005-1013 replaced with two glycinePromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-865)
Plasmid#203335PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
Tags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-865 onlyAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-885)
Plasmid#203334PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
Tags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-885 onlyAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a- phage MazG-like/Atd1
Plasmid#195244PurposeExpersses phage MazG-like/Atd1DepositorInsertphage MazG
TagsFlag and TwinStrepExpressionBacterialMutationCatalytic site mutantPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-20b_HtrA2(delta(133))_S306A_6His
Plasmid#197975PurposeE. coli expression of HtrA2(delta133)-6HisDepositorAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-A602E_M
Plasmid#146964PurposeBacterial Expression of NcQde-2_506-938-A602EDepositorInsertNcQde-2_506-938-A602E
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-H609W_M
Plasmid#146965PurposeBacterial Expression of NcQde-2_506-938-H609WDepositorInsertNcQde-2_506-938-H609W
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-K634A_M
Plasmid#146966PurposeBacterial Expression of NcQde-2_506-938-K634ADepositorInsertNcQde-2_506-938-K634A
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-R895A_M
Plasmid#146967PurposeBacterial Expression of NcQde-2_506-938-R895ADepositorInsertNcQde-2_506-938-R895A
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-Y595L_M
Plasmid#146968PurposeBacterial Expression of NcQde-2_506-938-Y595LDepositorInsertNcQde-2_506-938-Y595L
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-HsAGO2-448-859-optim_K
Plasmid#146729PurposeBacterial Expression of HsAGO2_448-859-optimDepositorInsertHsAGO2_448-859-optim (AGO2 Human)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmEDC3_331-400-ADA_H
Plasmid#146447PurposeBacterial Expression of DmEDC3_331-400-ADADepositorInsertDmEDC3_331-400-ADA (Edc3 Fly)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO5(wt)
Plasmid#191803PurposeFull length wt AtPCO5 with Nt-His tagDepositorInsertAtPCO5
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only