Addgene

We narrowed to 391 results for: SK2

Showing: 211 - 240 of 391 results

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pAB46 (CK2beta)

Plasmid

#27085

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-ER-KRAS(G12V)

Plasmid

#35635

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

ER

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4A(G12V)

Plasmid

#35634

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4A(G12V) (KRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pCGN K-Ras 12V, 188L

Plasmid

#14722

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

K-ras (KRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

12V, 188L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
RPS6KA4 gRNA (BRDN0001487122)

Plasmid

#77919

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA4 (RPS6KA4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA4 gRNA (BRDN0001146216)

Plasmid

#77920

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA4 (RPS6KA4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA4 gRNA (BRDN0001149050)

Plasmid

#77921

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA4 (RPS6KA4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SRPK2 gRNA (BRDN0001145577)

Plasmid

#75599

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK2 (SRPK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SRPK2 gRNA (BRDN0001145583)

Plasmid

#75600

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK2 (SRPK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SRPK2 gRNA (BRDN0001146940)

Plasmid

#75601

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK2 (SRPK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Use

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA2 gRNA (BRDN0001148851)

Plasmid

#75734

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA2 (RPS6KA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA2 gRNA (BRDN0001147334)

Plasmid

#75732

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA2 (RPS6KA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FKBP:mCherry-KRas4Bc30

Plasmid

#72900

Purpose

Membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

GTPase K-Ras (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158..188 (fragment 1..157 deleted)

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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KRAS-P34R

Plasmid

#158527

Purpose

Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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RPS6KA2 gRNA (BRDN0001149230)

Plasmid

#75735

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA2 (RPS6KA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TAOK1 gRNA (BRDN0001148505)

Plasmid

#76806

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAOK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAOK1 (TAOK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPc1 K-RAS 4A

Plasmid

#236451

Purpose

transient overexpression of K-RAS 4A in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4A (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MAP4K6

Plasmid

#229401

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K6 (MINK1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-KRas-Q61L

Plasmid

#214300

Purpose

Express constitutively active KRas fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active KRas (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-Q61L

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-KRas-S17N

Plasmid

#214301

Purpose

Express dominant negative KRas fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative KRas (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-S17N

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TAOK1 gRNA (BRDN0001145897)

Plasmid

#76807

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAOK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAOK1 (TAOK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TAOK1 gRNA (BRDN0001147719)

Plasmid

#76808

Purpose

3rd generation lentiviral gRNA plasmid targeting human TAOK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TAOK1 (TAOK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
RPS6KA2 gRNA (BRDN0001148104)

Plasmid

#75733

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA2 (RPS6KA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V constituitively activated

Promoter

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

Plasmid

#120562

Purpose

Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge