We narrowed to 2,369 results for: puc19
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Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
ExpressionBacterialMutationNAAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in VP2)
Plasmid#70032PurposepUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding regionDepositorInsertTSV full genome, linearized in VP2 gene
UseCloning vectorExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 ACMV DNA-B 1.5mer
Plasmid#159135PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 delta gap
Plasmid#49091PurposeTemplate for generating gene 60 mRNA, coding gap deletionDepositorInsertBacteriophage T4 gene 60
MutationCoding gap and coding gap STOP have been deleted.PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19::ZmUBI-RWT4Ca_K217R/K327R
Plasmid#237511PurposeProtoplast expressionDepositorInsertRwt4Ca_K217R/K327R
ExpressionPlantMutationMutations at kinase active sites (K217R, K327R)Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-HP1a-repair-template
Plasmid#238248PurposeFor knock-in msfGFP to HP1a locusDepositorInsertHP1a
UseCRISPRAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-TCOF1-repair-template
Plasmid#237632PurposeFor knock-in msfGFP to TCOF1 locusDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-FUS-repair-tempate
Plasmid#237684PurposeFor knock-in msfGFP to FUS locusDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19::ZmUBI-RWT4Ca+C-Tail
Plasmid#237514PurposeProtoplast expressionDepositorInsertRwt4Ca+C-Tail
ExpressionPlantMutationAdd C-terminal extensionAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-Bs3-sgRNA (BsaI)
Plasmid#188280PurposeMammalian expression, Genome editingDepositorInsertBs3-sgRNA
UseCRISPRExpressionMammalianMutationnonePromoterU6Available SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-3'
Plasmid#110406PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2DepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
ExpressionBacterialMutationshorter branch to 3' splice site distanceAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) AcAc
Plasmid#111332PurposeT7 transcriptionDepositorInsertACT1
ExpressionBacterialMutation3' splice site AcAcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pre-Ubc4 BrG pUC19
Plasmid#111322Purposefor transcription to Ubc4 pre-mRNADepositorInsertUBC4 (UBC4 Budding Yeast)
ExpressionBacterialMutationintron has branch-site A to G mutationAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap
Plasmid#49160PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gapDepositorInsertBacteriophage T4 gene 60
Mutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap + Cys
Plasmid#49159PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap + CysDepositorInsertBacteriophage T4 gene 600
MutationCoding gap and downstream sequence have been dele…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) A259C
Plasmid#111329PurposeT7 transcriptionDepositorInsertACT1
ExpressionBacterialMutationbranch site A259CAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) C256A
Plasmid#111331PurposeT7 transcriptionDepositorInsertACT1
ExpressionBacterialMutationbranch site C256AAvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2 GFP-P2A-BFP
Plasmid#202195Purposeknock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2DepositorAvailable SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-eGFP-P2A-dTAG-Ing3
Plasmid#170754PurposeDonor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locusDepositorInserteGFP-P2A-2xHA-dTAG
UseMouse TargetingTagseGFP-P2A-2xHA-dTAGMutationPhenylalanine 36 to valine in FKBP12PromoternoneAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-dual-scafffold-2.0 (pBP49)
Plasmid#218931PurposeThe dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.DepositorInsertdual scaffolds for CRISPR
UseCRISPRAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-703pUC19-tNGFR-P2A-BACH2
Plasmid#186112PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-701pUC19-tNGFR-P2A-FOXP3
Plasmid#186114PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-704pUC19-tNGFR-P2A-STAT3
Plasmid#186102PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only