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We narrowed to 17,659 results for: URE

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Showing: 351 - 400 of 17659 results

  4. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  6. SV.signed

    Plasmid
    #133933
    Purpose
    To validate the use of digital signatures in DNA.
    Depositor
    Jean Peccoud
    Insert
    lacZ and digital signature cassette
    Expression
    Bacterial
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133933-plasmid-map-sequence-id-268619

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  7. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64710-plasmid-map-sequence-id-108923

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  8. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  10. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  11. pAIP4_5IHW

    Plasmid
    #234148
    Purpose
    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5IHW
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234148-plasmid-map-sequence-id-464114

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  12. pAIP4_2OKT

    Plasmid
    #234149
    Purpose
    Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OKT
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234149-plasmid-map-sequence-id-464115

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  13. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  14. pJK322

    Plasmid
    #73494
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Promoter
    n/a
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73494-plasmid-map-sequence-id-135746

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  15. pJK684

    Plasmid
    #71638
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71638-plasmid-map-sequence-id-131658

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  16. pJK685

    Plasmid
    #71639
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71639-plasmid-map-sequence-id-131659

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  17. pJK687

    Plasmid
    #71640
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71640-plasmid-map-sequence-id-131661

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  18. pJK688

    Plasmid
    #71641
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71641-plasmid-map-sequence-id-131662

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  19. pJK695

    Plasmid
    #71644
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71644-plasmid-map-sequence-id-131665

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  20. pJK696

    Plasmid
    #71645
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71645-plasmid-map-sequence-id-131666

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  21. pJK607

    Plasmid
    #71626
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71626-plasmid-map-sequence-id-131645

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  22. pJK617

    Plasmid
    #71627
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71627-plasmid-map-sequence-id-131646

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  23. pJK643

    Plasmid
    #71628
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71628-plasmid-map-sequence-id-131647

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  24. pJK644

    Plasmid
    #71629
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71629-plasmid-map-sequence-id-131648

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  25. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  26. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

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  30. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  31. pJK692

    Plasmid
    #71643
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan, serine-38 to …
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71643-plasmid-map-sequence-id-131664

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  32. pJK653

    Plasmid
    #71630
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine, phenylalanine-79 …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71630-plasmid-map-sequence-id-131649

    Enlarge

  33. pJK654

    Plasmid
    #71631
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine, phenylalanine-79 to…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71631-plasmid-map-sequence-id-131651

    Enlarge

  34. pJK655

    Plasmid
    #71632
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate, phenylalanine-79 …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71632-plasmid-map-sequence-id-131652

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  35. pJK656

    Plasmid
    #71633
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine, phenylalanine-79 to …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71633-plasmid-map-sequence-id-131653

    Enlarge

  36. pJK659

    Plasmid
    #71634
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine, phenylalanine-79 t…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71634-plasmid-map-sequence-id-131654

    Enlarge

  37. pJK660

    Plasmid
    #71635
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71635-plasmid-map-sequence-id-131655

    Enlarge

  38. pJK661

    Plasmid
    #71636
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine, phenylalanine-79 t…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71636-plasmid-map-sequence-id-131656

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  39. pJK662

    Plasmid
    #71637
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan, cysteine-…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71637-plasmid-map-sequence-id-131657

    Enlarge

  40. pJK689

    Plasmid
    #71642
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71642-plasmid-map-sequence-id-131663

    Enlarge

  41. pJK697

    Plasmid
    #71646
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan, serine-38 to…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71646-plasmid-map-sequence-id-131667

    Enlarge

  42. pJK582

    Plasmid
    #71618
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala mutant (AaPurE1-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to alanine, additional N-termin…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71618-plasmid-map-sequence-id-131633

    Enlarge

  43. pJK583

    Plasmid
    #71619
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys mutant (AaPurE1-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to cysteine, additional N-termi…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71619-plasmid-map-sequence-id-131637

    Enlarge

  44. pJK584

    Plasmid
    #71620
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr mutant (AaPurE1-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to threonine, additional N-term…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71620-plasmid-map-sequence-id-131638

    Enlarge

  45. pJK608

    Plasmid
    #71621
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to aspartate, additional N-term…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71621-plasmid-map-sequence-id-131639

    Enlarge

  46. pJK677

    Plasmid
    #71623
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val mutant (AaPurE1-S57V)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to valine, additional N-termina…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71623-plasmid-map-sequence-id-131640

    Enlarge

  47. pJK686

    Plasmid
    #71624
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asn mutant (AaPurE1-S57N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to asparagine, additional N-ter…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71624-plasmid-map-sequence-id-131641

    Enlarge

  48. pJK657

    Plasmid
    #71647
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-40 to alanine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71647-plasmid-map-sequence-id-131642

    Enlarge

  49. pJK658

    Plasmid
    #71648
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-40 to asparagine, phenylalanine…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71648-plasmid-map-sequence-id-131644

    Enlarge

  50. pJK538

    Plasmid
    #72445
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72445-plasmid-map-sequence-id-133868

    Enlarge

Showing: 351 - 400 of 17659 results