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ApoE3_D125I_mCh-SspB (pBS1145)

Plasmid

#185327

Purpose

For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I

Use

Tags

Expression

Mammalian

Mutation

D125I

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_mCh-SspB (pBS1143)

Plasmid

#185325

Purpose

For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E2_255-317 (pBS0848)

Plasmid

#185280

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E2_255-317. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E2_255-317

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E2_47-300 (pBS0846)

Plasmid

#185278

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E2_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E2_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_52-358 (pBS0744)

Plasmid

#185235

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_52-358

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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S2 1058 APOBEC3 no UGI

Plasmid

#135350

Purpose

Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-rAPOBEC3-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP APOBEC3 no UGI

Plasmid

#135352

Purpose

Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

rAPOBEC3-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.1

Plasmid

#121903

Purpose

Plasmid for making zebrafish apoA-IVb.1 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.1 (apoa4b.1 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.3

Plasmid

#121905

Purpose

Plasmid for making zebrafish apoA-IVb.3 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoa4b.3 (apoa4b.3 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVa

Plasmid

#121902

Purpose

Plasmid for making zebrafish apoA-IVa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA4a (apoa4a Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.2

Plasmid

#121904

Purpose

Plasmid for making zebrafish apoA-IVb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.2 (apoa4b.2 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Perox-mTurq2-Apollo-NADP+

Plasmid

#193304

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Use

Tags

mTurqoise2, peroxisomal targeting sequence

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mCherry-ApoB-TagBFP

Plasmid

#129383

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

Depositor

Silvestro Conticello

Article

Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

Insert

mCherry-ApoB-Tag-BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Ef1alpha

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E3-T2A-mCherry

Plasmid

#190808

Purpose

Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E3

Promoter

Ubi C

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E4-T2A-mCherry

Plasmid

#190809

Purpose

Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E4

Promoter

Ubi C

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E2-T2A-mCherry

Plasmid

#190807

Purpose

Express APOE E2 allele from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E2

Promoter

Ubi C

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1 R33A

Plasmid

#138345

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1 R33A

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1 R33A

Use

Synthetic Biology

Tags

BP-NLS

Expression

Mutation

Change of Arg 33 to Ala.

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

Plasmid

#163562

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

Plasmid

#163561

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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