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pRS414-59

Plasmid

#23087

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Alanine, H3 S10A.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228374

Purpose

Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228381

Purpose

Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

N-terminal His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_OC43_NSP14

Plasmid

#213519

Purpose

Expresses NSP14 of hCoV OC43

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV OC43 NSP14 (ORF1ab hCoV OC43)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_NL63_NSP14

Plasmid

#213520

Purpose

Expresses NSP14 of hCoV NL63

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV NL63 NSP14 (ORF1ab hCoV NL63)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_229E_NSP14

Plasmid

#213521

Purpose

Expresses NSP14 of hCoV 229E

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV 229E NSP14

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET_SynPspA

Plasmid

#223812

Purpose

Bacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Use

Tags

10xHis and NusA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K

Plasmid

#194522

Purpose

Low copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N

Plasmid

#194524

Purpose

Low copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42N

Plasmid

#194525

Purpose

High copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H

Plasmid

#194526

Purpose

Low copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H

Plasmid

#194527

Purpose

High copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge