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pXZ13lacZalpha

Plasmid

#160229

Purpose

places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

Depositor

Ian Duncan

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNDGG005

Plasmid

#231309

Purpose

Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG006

Plasmid

#231310

Purpose

Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG007

Plasmid

#231311

Purpose

Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG021

Plasmid

#231305

Purpose

Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG002

Plasmid

#231308

Purpose

Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG001

Plasmid

#231307

Purpose

Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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L0I80_Bxb1mut(noBsaI)_GG1-GG4

Plasmid

#219715

Purpose

Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

Depositor

Jennifer Nemhauser

Insert

GG1-Bxb1(noBsaI0)-GG4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pNDGG010

Plasmid

#231315

Purpose

Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG022

Plasmid

#231306

Purpose

Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG009

Plasmid

#231314

Purpose

Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE104

Plasmid

#120744

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_EYK300-3AB

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GGE085

Plasmid

#120738

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_Tef

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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LII destination

Plasmid

#104529

Purpose

LII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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GGE001

Plasmid

#120740

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_PGM

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE083

Plasmid

#120732

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_URA3)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

Marker_URA3

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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