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hSyn-BiPOLES-mCerulean

Plasmid

#154944

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mCerulean3

Plasmid

#127592

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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hSyn-somBiPOLES-mCerulean

Plasmid

#154945

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSN001 pME minprom_mCerulean

Plasmid

#195959

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Alexa Burger

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter mCerulean

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCK002 pME mCerulean

Plasmid

#195956

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Alexa Burger

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mCerulean

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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mCerulean3-mVenus-CASPER

Plasmid

#58162

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mCerulean3 and mVenus-CASPER

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-XL-s2193

Plasmid

#166749

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL (BCL2L1 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean-PCNA-HygroR

Plasmid

#215064

Purpose

Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean3-BclXL-pEGFP-C1

Plasmid

#177408

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mDlx-SynTagMA_post-2A-mCerulean

Plasmid

#130899

Purpose

Synaptic Tag for Mapping Activity in GABAergic neurons

Depositor

Simon Wiegert

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA

Use

AAV

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Mcl-1-pLVX

Plasmid

#166752

Purpose

Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Mcl-1 (MCL1 Human)

Use

Lentiviral

Tags

mCerulean3

Expression

Mutation

Promoter

CMV

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-mCerulean

Plasmid

#154948

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

CaMKIIa(0.4)

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCerulean-PA-Rac1

Plasmid

#22030

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Tags

6X His and mCerulean

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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mDlx-BiPOLES-mCerulean

Plasmid

#154946

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

minimal Dlx

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCK081 desmaMCS:minpromCerulean,exorh:mCherry

Plasmid

#200014

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge