We narrowed to 1,516 results for: pet-28a

1. pET28a-DDX43

   Plasmid

   #134574

   Purpose

   Express human DDX43 protein (WT, full-length) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43 (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length WT

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-PHGDH

   Plasmid

   #74113

   Purpose

   Expression of N-terminal His-tagged

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   His-tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)- PC-MjMAT

   Plasmid

   #179372

   Purpose

   PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

   Depositor

   Andrea Rentmeister

   Article

   Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

   Insert

   PC-MjMAT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L147A, I351A

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-T4uvsW

   Plasmid

   #228201

   Purpose

   Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

   Depositor

   Stephen Benkovic

   Article

   Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

   Insert

   uvsW
   

   Use

   Tags

   hexahistidine and 10 amino acid linker

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Cut-P(11)4

    Plasmid

    #185785

    Purpose

    Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Cut-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-B4E

    Plasmid

    #162067

    Purpose

    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

    Depositor

    Karen Polizzi

    Article

    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

    Insert

    ampR-eIF4E fusion protein
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    eIF4E incorporating the K119A mutation, which is …

    Promoter

    T7

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-clbK

    Plasmid

    #114152

    Purpose

    Expresses ClbK

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbK
    

    Use

    Tags

    6xhis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-clbH-A1

    Plasmid

    #114149

    Purpose

    Expresses ClbH-A1

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbH-A1
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Contains only the A1 domain of ClbH

    Promoter

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-C9ORF80

    Plasmid

    #128418

    Purpose

    Express C9ORF80 in E. coli with a His tag (C-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    C9ORF80 (INIP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Human _TFAM_NoMTS_Dimer_pET28a+

    Plasmid

    #60013

    Purpose

    Expresses TFAM No MTS dimerization mutant in bacterial cells

    Depositor

    David Chan

    Article

    Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

    Insert

    Human TFAM No MTS (TFAM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Missing 1st 42 aas (Mitochondrial targeting seque…

    Promoter

    T7

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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