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pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Expression

Mutation

Promoter

Pfcrt

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfhsp86

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLyn-FRB-mCherry-nmd3-BSD

Plasmid

#85796

Purpose

Expression of PPM mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PPM Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfnmd3

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Article

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Use

Tags

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSLI-N-sandwich-loxP(K13)

Plasmid

#85793

Purpose

P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

double 2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-2xFKBP-GFP(1463000)

Plasmid

#85789

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R

Use

P. falciparum integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-Cdc45HA

Plasmid

#211427

Purpose

tet-inducible mammalian expression of human Cdc45 C-terminally fused to HA-Tag

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 OAS2-V5 puro

Plasmid

#158642

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS2 (OAS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 MX1-V5 puro

Plasmid

#158640

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged MX1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

Mx1 (MX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Venus-pLenti6

Plasmid

#36948

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

Venus (EYFP)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

-F46L/F64L/V68L/M153T/V163A/S175G

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-Drp1

Plasmid

#45160

Depositor

Richard Youle

Article

Frank et al Dev Cell. 2001 Oct;1(4):515-25.

Insert

Drp1 (DNM1L Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLX302 OASL-V5 puro

Plasmid

#158643

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OASL.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OASL (OASL Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 OAS1-V5 puro

Plasmid

#158641

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS1 (OAS1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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