We narrowed to 13,228 results for: sequence
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Plasmid#228423PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of E224-G243Available SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_K264-P282
Plasmid#228425PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of K264-P282Available SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9G_linker
Plasmid#228413PurposeExpression of NIT-9G with linkage region at the C terminus in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of the C terminal E domainAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_linker_nit9E
Plasmid#228412PurposeExpression of NIT-9E with linkage region at the N terminus in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaMutationtruncation of the N terminal G domainAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
UCT1m
Plasmid#121041PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertUCT-part guide RNA (Stanley Qi sequence)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertMitochondrially coded Arg8 protein
MutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
Tags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFosill-1
Plasmid#89695Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-3
Plasmid#89697Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-2
Plasmid#89696Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-4
Plasmid#89698Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone
Plasmid#164266PurposesgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.DepositorTypeEmpty backboneUseCRISPRExpressionWormPromoterU6 promoter from W05B2.8Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 V5-DEST mir-1-1 reporter
Plasmid#46646Purposeexpression of query pri-miRNADepositorInsertmir-1-1 reporter
UseRNAi; Expression of query pri-mirna sequences in …TagsV5ExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
Tags6xHis, thrombinExpressionBacterialAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
Tags6xHis, thrombinExpressionBacterialAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB2890
Plasmid#193133PurposeA2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.DepositorInsertGB_SynP (A2) G1dG2b.1
UseSynthetic BiologyAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJF449_dpy-10_CDS_sg1
Plasmid#163866PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF496_dpy-10_CDS_sg6
Plasmid#164267PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF495_dpy-10_CDS_sg6
Plasmid#164268PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterU6 promoter from W05B2.8Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only