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Lenti-eCas9-sgMIB1

Plasmid

#140239

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

sgRNA targeting human MIB1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Use

Tags

HIS-HA-CMYC

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-Emerald-WPRE-UbC-mCherry

Plasmid

#211802

Purpose

Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Inserts

Emerald
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV and UbC

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-mCherry-WPRE-UbC-Emerald

Plasmid

#211797

Purpose

Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Inserts

mCherry
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV and UbC

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL5-pCMV7.1

Plasmid

#155024

Purpose

N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 5 (CUL5 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL2-pCMV7.1

Plasmid

#155020

Purpose

N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 2 (CUL2 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mcherry p62 delta UBA silent

Plasmid

#187984

Purpose

mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Lacking UBA domain (aa389-434) but it has the sil…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4A-pCMV7.1

Plasmid

#155022

Purpose

N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4A (CUL4A Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL7-pCMV7.1

Plasmid

#155025

Purpose

N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 7 (CUL7 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

Plasmid

#175752

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y49A L50A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

Plasmid

#214469

Purpose

Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Inserts

ZFYVE27 (ZFYVE27 Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hHP1BP3-HA

Plasmid

#165468

Purpose

Human HP1BP3 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9

Plasmid

#140237

Purpose

Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

EF-1a core

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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