We narrowed to 2,378 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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1. pR26 CAG/BFP Dest

   Plasmid

   #74282

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFPB5A

   Plasmid

   #83556

   Purpose

   Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   GAL1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-EF1adCas9VP64T2ABsd-W

   Plasmid

   #112919

   Purpose

   Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9VP64-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Promoter

   FCP and U6

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PlepA

   Plasmid

   #55175

   Purpose

   Strong constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   lepA promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. PTEF1-yEGFPCLN2PEST-pRS406

   Plasmid

   #64406

   Purpose

   TEF promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   TEF1 promoter (TEF1 Budding Yeast)
   

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. amilCP chromoprotein promoter-less

   Plasmid

   #118008

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pShuttle

    Plasmid

    #192871

    Purpose

    Gateway compatible vector with BsaI cloning site

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Available Since

    Dec. 12, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28a-TS2126 RnlA-Strep

    Plasmid

    #159350

    Purpose

    Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

    Depositor

    Takashi Ito

    Article

    Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

    Insert

    TS2126 RNA ligase
    

    Tags

    His6 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. BB1_12_pAOX1

    Plasmid

    #98514

    Purpose

    BB1 with insert: promoter PAOX1

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    AOX1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SZ_EC_02

    Plasmid

    #155341

    Purpose

    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

    Depositor

    Jens Nielsen

    Article

    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

    Inserts

    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    

    Use

    Easycloneyali insert vector

    Expression

    Yeast

    Promoter

    gapdh, pyk1, and tef1

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RAMA-bio-His

    Plasmid

    #50737

    Purpose

    Expresses enzymatically monobiotinylated full length RAMA ectodomain

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RAMA
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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