We narrowed to 2,364 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Tags

   His6 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC102

   Plasmid

   #118883

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AsCas12a-Plus-mCherry

   Plasmid

   #191231

   Purpose

   To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   AsCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Mutation

   E174R, R951K, R955A

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PTEF1-yEGFPCLN2PEST-pRS406

   Plasmid

   #64406

   Purpose

   TEF promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   TEF1 promoter (TEF1 Budding Yeast)
   

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

   Plasmid

   #89693

   Purpose

   Luciferase reporter plasmid targeted at csr-1 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gsy-1 promoter, luciferase
   

   Use

   Yeast episomal vector

   Tags

   luciferase

   Promoter

   gsy-1

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SZ_EC_02

   Plasmid

   #155341

   Purpose

   Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

   Depositor

   Jens Nielsen

   Article

   Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

   Inserts

   tef1 promoter - xylulose kinase - pex20 terminator
   pyk1 promoter - xylose reductase - pex16 terminator
   gapdh promoter - xylitol dehydrogenase - lip2 terminator
   

   Use

   Easycloneyali insert vector

   Expression

   Yeast

   Promoter

   gapdh, pyk1, and tef1

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS4S

   Plasmid

   #55170

   Purpose

   Empty vector, integration at thrC, ampr, specr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. p15a_Kan_MLS_Rosa26_Short_CRISPRDel

   Plasmid

   #97008

   Purpose

   Rosa26 locus targeting vector

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Promoter

    pU6 5'CMV and EF1-alpha

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-CAG-IntCCCre-T2A-CopGFP

    Plasmid

    #160501

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL302-HA-SUMO2

    Plasmid

    #235497

    Purpose

    Expression of HA-SUMO2

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    SUMO2 (SUMO2 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. WN10151

    Plasmid

    #80441

    Purpose

    Expresses human codon-optimized dead (832A) LbCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Insert

    dead (D832A) LbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-GFP ArgSen

    Plasmid

    #184704

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKESaPar

    Plasmid

    #211829

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ePPEmax*

    Plasmid

    #205244

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQStrep2-PREI3

    Plasmid

    #31592

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    preimplantation protein 3 (MOB4 Human)
    

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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