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pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTL370

Plasmid

#69880

Purpose

attB flanked genomic Cirl for integration at attP sites.

Depositor

Tobias Langenhan, Robert Kittel

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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gMUM2-Citrine

Plasmid

#181965

Purpose

MUM2 protein fused to Citrine, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 (MUM2 Mustard Weed)

Use

Tags

Citrine YFP

Expression

Plant

Mutation

Promoter

MUM2

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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MUM2SP-Citrine

Plasmid

#181963

Purpose

MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 signal peptide, fused to the Citrine YFP in pAD

Use

Tags

Citrine YFP

Expression

Plant

Mutation

Promoter

MUM4pro 1.5kb fragment

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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L1-Ck1-hs1-l-chlo

Plasmid

#163937

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region left homologous sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs1-r-chlo

Plasmid

#163940

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region right homologous sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs2-r-chlo

Plasmid

#163941

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region right homologous sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_MpU6

Plasmid

#136120

Purpose

Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpU6

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12-MpER-Targ

Plasmid

#136094

Purpose

CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

ER targeting peptide from predicted chitinase Mapoly0069s0092

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_eGFP-GHtag-HDEL

Plasmid

#136096

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170812

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-GBdm

Plasmid

#129690

Purpose

express E. coli GreB E82C/C68S

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GreB E82C C68S (greB E. coli)

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPN158

Plasmid

#91687

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN159

Plasmid

#91688

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN220

Plasmid

#91681

Purpose

Express sgRNA targeting human VRK2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

VRK2 (VRK2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN221

Plasmid

#91682

Purpose

Express sgRNA targeting human VRK2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

VRK2 (VRK2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN154

Plasmid

#91683

Purpose

Express sgRNA targeting human ZNF536

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZNF536 (ZNF536 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge