We narrowed to 3,098 results for: actin

1. pCAG-Cas13d-T2A-GFP(1-10)

   Plasmid

   #224566

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-GFP(1-10)
   

   Use

   CRISPR

   Tags

   T2A-split GFP(1-10)

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI H2B-GFP

   Plasmid

   #92399

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

   Plasmid

   #229782

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   3x mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAct:dCas9-GCN4

   Plasmid

   #78899

   Purpose

   Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-10XGCN4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lifeact-μDpTT

   Plasmid

   #182322

   Purpose

   Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lifeact-μDronpa-TagRFP-T
   

   Use

   Tags

   N-terminal 17 amino acids from budding yeast Abp1…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos4b-Lifeact-7

   Plasmid

   #54813

   Purpose

   Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mEos4b-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pIS1-Actb5UTR-renilla

   Plasmid

   #38234

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Actb 5'UTR / Renilla (Actb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Actb 5' UTR fused to renilla ORF

   Promoter

   Endogenous Actb

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

9. mEos2-FilaminA-N-9

   Plasmid

   #57379

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   FilaminA (FLNA Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

    Plasmid

    #53959

    Purpose

    allows Cre-mediated expression of transgene

    Depositor

    Jeffrey Robbins

    Article

    Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Lox-CAT-Lox

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer, chicken actin promoter

    Available since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR008_APAD

    Plasmid

    #204818

    Purpose

    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    

    Use

    Tags

    HA and Flag and huADAR2dd

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBa-FRB-3myc-Rab5a

    Plasmid

    #64209

    Purpose

    Rab5a with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Rab5a (Rab5a Mouse)
    

    Use

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    chicken Beta Actin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB.FRB-3myc-KLC1a

    Plasmid

    #134627

    Purpose

    Mammalian expression of mouse KLC1a

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KLC1a (Klc1 Mouse)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAP Z alpha 1 beta 1 CP

    Plasmid

    #13451

    Depositor

    John Cooper

    Article

    Soeno et al J Muscle Res Cell Motil. 1998 Aug . 19(6

    Insert

    capping protein alpha 1 beta 1 (chicken) (CAPZB Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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