We narrowed to 3,255 results for: actin

1. mEos2-Fascin-C-10

   Plasmid

   #57376

   Purpose

   Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMADM-beta

   Plasmid

   #36891

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   Beta-Geo
   thymidine kinase
   

   Expression

   Mammalian

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLifeAct_mScarlet-H_N1

   Plasmid

   #85055

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xmTurquoise2_UtrCH

   Plasmid

   #112963

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   UtrCH
   

   Tags

   3x mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Bgh-GFP-Farpl

   Plasmid

   #115501

   Purpose

   expression of rat Farp1

   Depositor

   Thomas Biederer

   Article

   Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

   Insert

   Farp1 (Farp1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   chicken beta-actin

   Available Since

   Dec. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-VinculinI997A_TurboID_Venus

   Plasmid

   #200274

   Purpose

   Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

   Depositor

   Brenton Hoffman

   Article

   Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

   Insert

   VinculinI997A_TurboID_Venus
   

   Expression

   Mammalian

   Mutation

   change isoleucine to alanine at 997 of vinculin

   Promoter

   T7

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Clover-Lifeact-7

   Plasmid

   #54551

   Purpose

   Localization: Actin, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   Clover

   Expression

   Mammalian

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. mTurquoise2-UtrCH

   Plasmid

   #98824

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   UtrCH
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LR (bact: HyPer)

    Plasmid

    #162069

    Purpose

    Used for the diffuse visualization of H2O2 production in cells.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    beta actin 2 (actb2 Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    HyPer

    Promoter

    T3

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniSOG-Lifeact-7

    Plasmid

    #54823

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Tags

    Lifeact and miniSOG

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pHyVec12

    Plasmid

    #51851

    Purpose

    Expression in Hydra for RNAi

    Depositor

    Haifan Lin

    Article

    Juliano et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Inserts

    GFP hairpin
    DsRed2
    

    Use

    RNAi; Hydra expression

    Promoter

    Actin

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. ITPKA_3xmTurquoise

    Plasmid

    #112961

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    ITPKA
    

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p35S::mCh-HA-P2A-lifeact-mCh

    Plasmid

    #135234

    Purpose

    constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    mCh-HA-P2A-lifeact-mCh
    

    Tags

    HA, lifeact, and p2a

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSR (mCherry-surrogateTarget-bsr)

    Plasmid

    #66950

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    blasticidin
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    beta-actin

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179055

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-ARC35-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. mTFP1-VASP-5

    Plasmid

    #55513

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    VASP
    

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. ReaLiSM A1-Hinge-GPIba+2cys

    Plasmid

    #59581

    Purpose

    express VWF A1-GPIba ReaLiSM construct in mammalian cells

    Depositor

    Timothy Springer

    Article

    Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Tags

    6His

    Expression

    Mammalian

    Promoter

    chick beta-actin

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAct:MCP-VP64

    Plasmid

    #78904

    Purpose

    Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2:VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    March 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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