We narrowed to 2,785 results for: cdna expression plasmid
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Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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STING-V1
Plasmid#124262PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
V2-STING
Plasmid#124265PurposeN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus2ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
V1-STING
Plasmid#124263PurposeN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus2ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH21-Tier1-PhEF1a
Plasmid#169561PurposeTier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA
Plasmid#67473PurposeMammalian expression of hSara with stop codon (native expression)DepositorInsertSARA (SAR1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1
Plasmid#67472PurposeMammalian expression of hSar1 with stop codon (native expression)DepositorInsertSAR1 (SAR1A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APP695
Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H127
Plasmid#170343Purpose6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH234-Tier1-PmPGK1-tTA
Plasmid#169574PurposeTier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorInsertSAR1 (SAR1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorInsertSAR1 (SAR1A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA-GFP
Plasmid#67410PurposeMammalian expression of hSara with C-term GFP tagDepositorInsertSARA (SAR1B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SARA-V5-His6
Plasmid#67452PurposeMammalian expression of hSara with C-term V5 and His6 tagDepositorInsertSARA (SAR1B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only