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pFA6a-mTID-HA3-HIS3MX6

Plasmid

#227983

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBCm

Plasmid

#46763

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTcI-Wasabi

Plasmid

#86101

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-rtn1a-KALTA4

Plasmid

#239989

Purpose

Expresses GAL4 under control of the rtn1a promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and rtn1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-TFAM

Plasmid

#166217

Purpose

Gateway entry vector for N. furzeri TFAM

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

TFAM

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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JARID1CA

Plasmid

#74651

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JARID1CA (KDM5C Human)

Use

Baculovirus

Tags

His-6-TEV

Promoter

PH

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-atp6v0cb-KALTA4

Plasmid

#239985

Purpose

Expresses GAL4 under control of the atp6v0cb promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

atp6v0cb and myl7

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Halo

Plasmid

#200197

Purpose

Expression for SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_psmyc_His6_triple red

Plasmid

#170047

Purpose

Mono-Responsive Reporter Gene Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-HIS3MX6

Plasmid

#228023

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-NLS-3HA-HISMX6

Plasmid

#228027

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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