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pMOD_A0802

Plasmid

#91023

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0201

Plasmid

#91002

Purpose

Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0202

Plasmid

#91003

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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GWF297

Plasmid

#133612

Purpose

TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJG488

Plasmid

#91196

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+gUbi1+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2517

Plasmid

#91074

Purpose

Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

TaU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2121

Plasmid

#91065

Purpose

Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

GmUbi

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-04

Plasmid

#166928

Purpose

STAR-DC part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Dual control activator -Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_252

Plasmid

#100504

Purpose

Mammalian expression of E. coli LF82_252 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_252

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_052

Plasmid

#100501

Purpose

Mammalian expression of E. coli LF82_052 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_052

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_351

Plasmid

#100491

Purpose

Mammalian expression of E. coli LF82_351 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_351

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_349

Plasmid

#100506

Purpose

Mammalian expression of E. coli LF82_349 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_349

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_441

Plasmid

#100498

Purpose

Mammalian expression of E. coli LF82_441 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_441

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_136

Plasmid

#100495

Purpose

Mammalian expression of E. coli LF82_136 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_136

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_702

Plasmid

#100473

Purpose

Mammalian expression of E. coli LF82_702 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_702

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_315

Plasmid

#100505

Purpose

Mammalian expression of E. coli LF82_315 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_315

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_468

Plasmid

#100502

Purpose

Mammalian expression of E. coli LF82_468 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_468

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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