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169,348 results

Showing: 7991 - 8000 of 169348 results

  1. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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  2. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100050
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    CaMKIIa
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100050-plasmid-map-sequence-id-194451

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  3. EF1α-mNeonGreen-Z1

    Plasmid
    #135613
    Purpose
    mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135613-plasmid-map-sequence-id-269406

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  5. pUC57-LbNOX

    Plasmid
    #75285
    Purpose
    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    LbNOX
    Tags
    C-terminal FLAG tag with linker
    Mutation
    Human codon optimized
    Available Since
    May 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    75285-plasmid-map-sequence-id-258257

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  9. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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Showing: 7991 - 8000 of 169348 results