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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-NES reporter (pDN160)

Plasmid

#72660

Purpose

NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_BE4max_P2A_GFP

Plasmid

#112099

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

Plasmid

#203965

Purpose

Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

ARID1A (Arid1a Mouse)

Use

Sleeping beauty transposon

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HA-DLC1-i4

Plasmid

#99534

Purpose

DLC1-isoform 4 expression plasmid

Depositor

Qian Tao

Article

Low et al Oncogene. 2011 Apr 21;30(16):1923-35. do

Insert

DLC1-isoform 4 (DLC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-mt4xi-GCaMP6f

Plasmid

#192075

Purpose

Fluorescent reporter for mitochondrial calcium

Depositor

Timothy Ryan

Insert

4 repeats of improved COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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HDAC6 Flag

Plasmid

#13823

Purpose

Mammalian expression of human histone deacetylase 6 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC6 (HDAC6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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