We narrowed to 2,387 results for: MEA

1. pcDNA3.1(+)Gα15-244-NLuc

   Plasmid

   #207154

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA15 (GNA15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position E(244)N

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TPC2-G-GECO1.2

   Plasmid

   #207141

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMRX-IBU-pSu9-HaloTag7-SNAP

   Plasmid

   #217319

   Purpose

   Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

   Depositor

   Noboru Mizushima

   Article

   Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

   Insert

   Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
   

   Use

   Retroviral

   Tags

   HaloTag and SNAP

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HP35 tension sensor

   Plasmid

   #101250

   Purpose

   Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-HP35-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pyronic/pcDNA3.1(-)

   Plasmid

   #51308

   Purpose

   Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

   Insert

   Pyronic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABE hygro MEN1 WT

   Plasmid

   #11024

   Depositor

   Matthew Meyerson

   Article

   Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

   Insert

   MEN1 (MEN1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212962

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_non-targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212963

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_GFP targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMax_mOrange_A215C

    Plasmid

    #177828

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_A215C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A215C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LVDP-CArG-RE-GPR

    Plasmid

    #89762

    Purpose

    Quantitative measurement of CArG response element activity.

    Depositor

    Stelios Andreadis

    Article

    Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

    Insert

    6X CArG-RE
    

    Use

    Lentiviral

    Tags

    CMV-ZsGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. F40 tension sensor

    Plasmid

    #101252

    Purpose

    Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-F40-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AEB-No-aptamer-controlmRFP1

    Plasmid

    #63848

    Purpose

    This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23100

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. stabilized HP35 tension sensor module

    Plasmid

    #101251

    Purpose

    Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35st-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFAB3677

    Plasmid

    #47833

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 2 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFAB3833

    Plasmid

    #47839

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 8 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFAB3737

    Plasmid

    #47838

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 7 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge