We narrowed to 5,102 results for: codon optimized

1. pENTR-ABCC1_K684I_STOP

   Plasmid

   #221430

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Insert

   ABCC1 (ABCC1 Human)
   

   Expression

   Mammalian

   Mutation

   K684I

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-ABCC1_S769N_STOP

   Plasmid

   #221431

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Insert

   ABCC1 (ABCC1 Human)
   

   Expression

   Mammalian

   Mutation

   S769N

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

   Plasmid

   #198022

   Purpose

   LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population

   Depositor

   Donald Kohn

   Insert

   CD3D c.202C>T-cDNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized (20 bp) on either end of cDNA for…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-c-MNDU3-CD3D-275-2A>G

   Plasmid

   #198024

   Purpose

   LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan

   Depositor

   Donald Kohn

   Insert

   CD3D c.275-2A>G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized (20 bp) on either end of cDNA for…

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103 UTROPHIN WW domain

   Plasmid

   #104275

   Purpose

   Bacterial expression of WW domain from UTROPHIN

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   UTROPHIN WW domain (UTRN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103 ARHGAP27 WW domain #1

   Plasmid

   #104282

   Purpose

   Bacterial expression of WW domain #1 from ARHGAP27

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   ARHGAP27 WW domain #1 (ARHGAP27 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103 ARHGAP27 WW domain #2

   Plasmid

   #104283

   Purpose

   Bacterial expression of WW domain #2 from ARHGAP27

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   ARHGAP27 WW domain #2 (ARHGAP27 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103 PDZK10 WW domain

   Plasmid

   #104285

   Purpose

   Bacterial expression of WW domain from PDZK10

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   PDZK10 WW domain (FRMPD4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 KIAA0082 WW domain

    Plasmid

    #104288

    Purpose

    Bacterial expression of WW domain from KIAA0082

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIAA0082 WW domain (CMTR1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103 DiGeorge WW domain

    Plasmid

    #104292

    Purpose

    Bacterial expression of WW domain from DiGeorge

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    DiGeorge WW domain (DGCR8 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103 FNBP4 WW domain #1

    Plasmid

    #104263

    Purpose

    Bacterial expression of WW domain #1 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #1 (FNBP4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103 FNBP4 WW domain #2

    Plasmid

    #104264

    Purpose

    Bacterial expression of WW domain #2 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #2 (FNBP4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103 DRP2 WW domain

    Plasmid

    #104270

    Purpose

    Bacterial expression of WW domain from DRP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    DRP2 WW domain (DRP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103 HECW2 WW domain #1

    Plasmid

    #104244

    Purpose

    Bacterial expression of WW domain #1 from HECW2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    HECW2 WW domain #1 (HECW2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAST4

    Plasmid

    #38998

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAST4 (MAST4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. MAST1

    Plasmid

    #39065

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAST1 (MAST1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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