We narrowed to 9,995 results for: pET

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET23b-proMMP-7

Plasmid

#29524

Depositor

William Parks

Publication

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Available since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

Plasmid

#48286

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and MBP

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET StrepII TEV co-transformation cloning vector (13K-R)

Plasmid

#48319

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Publication

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSG086

Plasmid

#214263

Purpose

PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

Depositor

Ute Romling

Publication

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M

Tags

6x His-tag

Expression

Bacterial

Mutation

substitution of 31 amino acids of FtsH2 (F124RRFA…

Promoter

T7 promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLz26_CsoS2_N1-N2_GFP

Plasmid

#140851

Purpose

His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeats 1-2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a Halotag-CNA35

Plasmid

#131128

Purpose

Halotag-CNA35 peptide for Collagen labelling

Depositor

Samantha Stehbens

Publication

Ju et al Nat Cell Biol. 2024 Sep;26(9):1520-1534.

Insert

Halotag-CNA35

Tags

Halotag

Expression

Bacterial

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Publication

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Publication

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

V266E

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-p65(19-325)

Plasmid

#44744

Depositor

Gourisankar Ghosh

Insert

p65 (Rela Mouse)

Expression

Bacterial

Mutation

AA 19-325

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETDuet-1-PX-Gal-3-TRUNC

Plasmid

#256297

Purpose

Expresses truncated PX-Gal-3 (deletion of N-terminal domain, aa 1-116) in bacterial cells

Depositor

Mia Huang

Publication

Joeh et al Proc Natl Acad Sci U S A. 2020 Nov 3;117

Insert

PX-Gal-3-TRUNC (LGALS3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Deleted residues 1-116

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-PX-Gal-3-MUT

Plasmid

#256296

Purpose

Expresses mutated PX-Gal-3 (R144S, R186S, G182A) in bacterial cells

Depositor

Mia Huang

Publication

Joeh et al Proc Natl Acad Sci U S A. 2020 Nov 3;117

Insert

PX-Gal-3-MUT (LGALS3 Human)

Tags

His tag

Expression

Bacterial

Mutation

mutatd R144S, R186S, G182A

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pET29b_His-TEV-SpyCatcher003-C

Plasmid

#233339

Purpose

To express SpyCatcher

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

His-TEV-SpyCatcher003-C

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH21-His

Plasmid

#233333

Purpose

To express nanobody VHH21 with N terminal SpyTag

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH21-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET24+ CUL5(1-384)

Plasmid

#246504

Purpose

Recombinant protein expression of CUL5(1-384)

Depositor

Steven Clarke

Publication

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

cullin-5 (CUL5 Human)

Expression

Bacterial

Mutation

Deleted amino acids 385-780

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-TGP-TwinStrep

Plasmid

#240251

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Publication

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, Thrombin, Twin-Strep, and thermostable G…

Expression

Bacterial

Promoter

T7

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-ALFA-TwinStrep

Plasmid

#240252

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Publication

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, ALFA, Thrombin, and Twin-Strep

Expression

Bacterial

Promoter

T7

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-Hf-RfxCas13d-His

Plasmid

#234480

Purpose

Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Changed four alanine to valine in positions 134, …

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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