We narrowed to 6,904 results for: crispr cas9 plasmids

1. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_PROX1_1+4

   Plasmid

   #121177

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-J23109

   Plasmid

   #113151

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. peSpCas9-J23109

   Plasmid

   #113152

   Purpose

   Plasmid constitutively expressing eSpCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   eSpCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K810A & K1003A & R1060A

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. peSpdCas9-J23109

   Plasmid

   #113153

   Purpose

   Plasmid constitutively expressing eSpdCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   eSpdCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & K810A & H840A & K1003A & R…

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

   Plasmid

   #140000

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-dHeFSpCas9

    Plasmid

    #92116

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    Cbh

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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