We narrowed to 31,520 results for: Eng

1. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

   Plasmid

   #182895

   Purpose

   Mammalian expression of PVALB rat mAb LC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) light chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag-SENP1

   Plasmid

   #17357

   Depositor

   Edward Yeh

   Article

   Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

   Insert

   SENP1 (SENP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-SENP1m

   Plasmid

   #17358

   Depositor

   Edward Yeh

   Article

   Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

   Insert

   SENP1 (SENP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R630L, K631M

   Promoter

   Available since

   March 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pH3U3-mcs

   Plasmid

   #12609

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

   Insert

   Multiple cloning site in His3 Ura3 reporter plasmid
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX408 Francisella tularensis subsp. novicida Cas9

   Plasmid

   #68705

   Purpose

   Francisella tularensis subsp. novicida Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCC2

   Plasmid

   #39993

   Depositor

   Paul Modrich

   Article

   Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

   Insert

   EcoRI endonuclease & EcoRI methylase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   n/a

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC1817_pCR8-gSMN2-EX

   Plasmid

   #118651

   Purpose

   Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-EX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Plasmid

   #68337

   Purpose

   Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Depositor

   Feng Zhang

   Insert

   St3Cas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EB2316

   Plasmid

   #87753

   Purpose

   pkaiBC::EYFP cloned between XbaI and XhoI of EB2065

   Depositor

   Erin O'Shea

   Article

   Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

   Insert

   pkaiBC::EYFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC1808_pmax-RBFOX1N-FRB-C

    Plasmid

    #118641

    Purpose

    Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-FRB-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC1809_pmax-RBM38-FRB

    Plasmid

    #118642

    Purpose

    Transient transfection; Expresses RBM38-FRB; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBM38-FRB
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC1819_pCR8-DR-SMN2-2-DR

    Plasmid

    #118653

    Purpose

    Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-SMN2-2-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC1810_pmax-FRB-RBM38

    Plasmid

    #118643

    Purpose

    Transient transfection; Expresses FRB-RBM38; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    FRB-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC1818_pCR8-gRG6-SA

    Plasmid

    #118652

    Purpose

    Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gRG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC1820_pCR8-DR-RG6-SA-DR

    Plasmid

    #118654

    Purpose

    Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-RG6-SA-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC1822_pCR8-dCasRx

    Plasmid

    #118656

    Purpose

    Gateway Donor; dCasRx ORF; No promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EB2315

    Plasmid

    #87752

    Purpose

    BbbJ23119::EYFP cloned between XbaI and XhoI of EB2065

    Depositor

    Erin O'Shea

    Article

    Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

    Insert

    BbbJ23119::EYFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX379 Legionella pneumophila str. Paris Cas9

    Plasmid

    #68316

    Purpose

    Legionella pneumophila str. Paris Cas9

    Depositor

    Feng Zhang

    Insert

    LpCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX391 Sphaerochaeta globus str. Buddy Cas9

    Plasmid

    #68328

    Purpose

    Sphaerochaeta globus str. Buddy Cas9

    Depositor

    Feng Zhang

    Insert

    SgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EB2317

    Plasmid

    #87754

    Purpose

    tetR-mTurqoise2 cloned between BamHI and EcoRI of pAM2991

    Depositor

    Erin O'Shea

    Article

    Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

    Insert

    TetR-mTurq2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVP311

    Plasmid

    #222070

    Purpose

    MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pVP312

    Plasmid

    #222071

    Purpose

    MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pVP313

    Plasmid

    #222072

    Purpose

    MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pVP314

    Plasmid

    #222073

    Purpose

    MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pVP315

    Plasmid

    #222074

    Purpose

    MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pVP316

    Plasmid

    #222075

    Purpose

    MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pVP078

    Plasmid

    #222030

    Purpose

    MultiGreen 2.0 AB level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pVP079

    Plasmid

    #222031

    Purpose

    MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pVP080

    Plasmid

    #222032

    Purpose

    MultiGreen 2.0 CD level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pVP081

    Plasmid

    #222033

    Purpose

    MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pVP082

    Plasmid

    #222034

    Purpose

    MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pVP083

    Plasmid

    #222035

    Purpose

    MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All unused Esp3i sites are domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pRSET A-mEosEM-E

    Plasmid

    #226336

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    mEosEM-E
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBAD-HaloKbp1a

    Plasmid

    #228793

    Purpose

    Chemigenetic indicator for potassium ion - high affinity

    Depositor

    Robert Campbell

    Article

    Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

    Insert

    HaloKbp1a
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD-HaloKbp1b

    Plasmid

    #228794

    Purpose

    Chemigenetic indicator for potassium ion - low affinity

    Depositor

    Robert Campbell

    Article

    Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

    Insert

    HaloKbp1b
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBAD-HaloKbp1c

    Plasmid

    #228795

    Purpose

    Chemigenetic indicator for potassium ion - medium affinity

    Depositor

    Robert Campbell

    Article

    Cheng et al J Am Chem Soc. 2024 Nov 27. doi: 10.1021

    Insert

    HaloKbp1c
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1_SV40-iBlue-pA

    Plasmid

    #200234

    Purpose

    Backbone for cloning iSBH-sgRNAs

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEM3/cGata1

    Plasmid

    #83829

    Purpose

    Cloning of chicken GATA1

    Depositor

    Douglas Engel

    Article

    Yamamoto et al Genes Dev. 1990 Oct;4(10):1650-62.

    Insert

    Gata1 (GATA1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFBOH-MHL_EML4:250-877

    Plasmid

    #210914

    Purpose

    Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EML4:I250-A877 (EML4 Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    K283E natural variant (VAR_031726), I382V Natural…

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pFBD-BirA_ATG16L1:303-607

    Plasmid

    #210942

    Purpose

    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ATG16L1:G303-Y607 (ATG16L1 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 4, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pFBD-BirA_LRRK2:2141-2527

    Plasmid

    #210901

    Purpose

    Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    LRRK2:T2141-E2527 (LRRK2 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    M2397T natural variant ( VAR_024965)

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pBacMam2-DiEx-LIC_LRRK2:2141-2527

    Plasmid

    #210900

    Purpose

    Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    LRRK2:T2141-E2527 (LRRK2 Human)
    

    Use

    Tags

    AENLYFQSHHHHHHHHHHDYKDDDDK and M

    Expression

    Insect

    Mutation

    M2397T natural variant ( VAR_024965)

    Promoter

    P10 and CMV

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pFBD-BirA_CDC20:161-P477

    Plasmid

    #210888

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pFBD-BirA_CDC40:236-578

    Plasmid

    #210885

    Purpose

    Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC40:E236-W578 (CDC40 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBiFC-bFosVC155

    Plasmid

    #22013

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Oct. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET29b_mNeonGreen

    Plasmid

    #209425

    Purpose

    Express mNeonGreen in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    mNeonGreen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. mEosEM-E_N1

    Plasmid

    #226337

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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