We narrowed to 3,255 results for: actin

1. Tg(mpeg1:LifeAct-mRuby)

   Plasmid

   #166977

   Purpose

   labels F-actin in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

   Inserts

   mpeg1 (mpeg1.1 Zebrafish)
   LifeAct (actb1 Frog)
   

   Tags

   Lifeact-mRuby and mRuby

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS SARS-CoV-2 XBB Spike

   Plasmid

   #195287

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 XBB Spike

   Depositor

   Marceline Côté

   Insert

   SARS-CoV-2 XBB Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24-27…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   Feb. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. mEos2-FilaminA-N-9

   Plasmid

   #57379

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   FilaminA (FLNA Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pExpreS2-2

   Plasmid

   #175362

   Purpose

   G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

   Plasmid

   #52889

   Purpose

   "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Insect

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dronpa-Lifeact-7

   Plasmid

   #54740

   Purpose

   Localization: Actin, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Article

   Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

   Type

   Empty backbone

   Tags

   Dronpa-Lifeact

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBa.GFP-KIF5B

   Plasmid

   #134625

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKate1.31-Lifeact-7

   Plasmid

   #54668

   Purpose

   Localization: Actin, Excitation: 588, Emission: 635

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Insert

   LifeAct
   

   Tags

   mKate1.31 (aka mKate2)

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pUB_smFLAG_ActB_MS2

   Plasmid

   #81083

   Purpose

   Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_ActB_24xMS2
   

   Expression

   Mammalian

   Available Since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pclbw-eGFPomp25

    Plasmid

    #58424

    Purpose

    Mammalian expression of GFP targeted to the mitochondrial outer membrane

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    eGFP-omp25 (Synj2bp Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa's 109-145 of mouse omp25

    Promoter

    CMV/b-actin

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. E407V Nuclear Myosin I YFP

    Plasmid

    #60242

    Purpose

    Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    E407V

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos4b-Lifeact-7

    Plasmid

    #54813

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4b-Lifeact

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pIS1-Actb5UTR-renilla

    Plasmid

    #38234

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Actb 5'UTR / Renilla (Actb Mouse)
    

    Expression

    Mammalian

    Mutation

    Actb 5' UTR fused to renilla ORF

    Promoter

    Endogenous Actb

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

    Plasmid

    #53959

    Purpose

    allows Cre-mediated expression of transgene

    Depositor

    Jeffrey Robbins

    Article

    Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Lox-CAT-Lox

    Expression

    Mammalian

    Promoter

    CMV enhancer, chicken actin promoter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Lifeact-μDpTT

    Plasmid

    #182322

    Purpose

    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-μDronpa-TagRFP-T
    

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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