We narrowed to 5,296 results for: cycs
-
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
SDCCAG10
Plasmid#25610PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSDCCAG10 (CWC27 Human)
UseTagsHisExpressionBacterialMutationCyclophilin_CeCYP16-Like Domain (residues 8-173)PromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF mito-PAcGFP
Plasmid#188905PurposeExpresses mitochondrial photoactivatable GFPDepositorInsertmitochondrial photoactivatable GFP
UseTagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
His8OphMA-21mer.
Plasmid#189670PurposeHis8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.DepositorInsertHis8OphMATEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
His8OphMADeltaC6-18mer.
Plasmid#189671PurposeHis8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptidesDepositorInsertHis8OphMA∆C6-TEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CylK-Strep
Plasmid#185962PurposeExpresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparationDepositorInsertCylK
UseTagsHis6-SumoExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-DRS2
Plasmid#177104PurposeExpresses iGFP-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB300-Tier1-PhCMV-rTetR
Plasmid#169577PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline repressor protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-AUR1-msGFP2
Plasmid#177101PurposeExpresses Aur1-msGFP2 in yeast cellsDepositorInsertAUR1 (AUR1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RBD2-6xmGFP
Plasmid#177109PurposeExpresses Rbd2-6xmEGFP in yeast cellsDepositorInsertRBD2 (RBD2 Budding Yeast)
UseTags6xmEGFPExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC2-msGFPx3
Plasmid#177110PurposeExpresses Sec2-msGFPx3 in yeast cellsDepositorInsertSEC2 (SEC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YSBE415: pTOP_ACA1_Vc_HOG1 terminator
Plasmid#122434PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE416: pTOP_CAC1_Vn_HOG1 terminator
Plasmid#122435PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMST-1313
Plasmid#118076PurposepTEF1-cexA-tCYC1DepositorInsertpTEF1-cexA-tCYC1
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Tet
Plasmid#104524PurposeLI resistance gene moduleDepositorInsertTetracycline resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only