166,548 results

1. pcDNA-mito-dGreenPy1

   Plasmid

   #239073

   Purpose

   Mammalian expression of mitochondria-targeted green fluorescent pyruvate control biosensor dGreenPy1

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   dGreenPy1
   

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

   Plasmid

   #124603

   Purpose

   Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   Soma-localized ChrimsonR
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

   Plasmid

   #225752

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA GSK3 beta S9A pcDNA3

   Plasmid

   #14754

   Purpose

   Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

   Depositor

   Jim Woodgett

   Article

   Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

   Insert

   GSK3 beta S9A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A. Constitutively active mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLV_hEF1a_rtTA3

   Plasmid

   #61472

   Purpose

   3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Insert

   rtTA3
   

   Use

   Lentiviral

   Promoter

   hEF1a

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR223-BALF4

   Plasmid

   #195835

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BALF4

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF4 (BALF4 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD454-SR mouse Alpha-synuclein

    Plasmid

    #89075

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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