166,548 results

1. HA-HIF1alpha-wt-pBabe-puro

   Plasmid

   #19365

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt human cDNA

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSNAP-CaaX Control Plasmid

   Plasmid

   #101133

   Purpose

   Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   CaaX Box
   

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRSF-MjCouRS

   Plasmid

   #229066

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   Methanococcus jannaschii tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV GFAP jGCaMP7b

   Plasmid

   #171118

   Purpose

   Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

   Depositor

   Thomas Oertner

   Article

   Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   6 His Tag, T7 Tag, and Xpress Tag

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.4-GFP-TEV-B55

   Plasmid

   #217378

   Purpose

   expresses GFP tagged B55 in mammalian cells; tev cleavable

   Depositor

   Wolfgang Peti

   Article

   Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

   Insert

   B55 (PPP2R2A Human)
   

   Tags

   EGFP TEV

   Expression

   Mammalian

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4-ERSE2-luc2P-Hygro

   Plasmid

   #101790

   Purpose

   Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-II
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   minimal TATA-box promoter with low basal activity

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Synapsin and U6

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetON-shYAP

   Plasmid

   #115667

   Purpose

   Inducible YAP knockdown

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP shRNA (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ph2b

   Plasmid

   #32109

   Depositor

   Christopher Buck

   Article

   Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

   Insert

   BKV VP2
   

   Expression

   Mammalian

   Mutation

   codon modified

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GGamma7-GFP2

    Plasmid

    #166778

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma7-GFP2 (GNG7 Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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