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CDK2 gRNA (BRDN0001148866)

Plasmid

#77191

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUDP013

Plasmid

#103873

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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MASTL gRNA (BRDN0001148591)

Plasmid

#75989

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MASTL (MASTL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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YES1 (T348I) in pLX303

Plasmid

#42564

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T348I

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
AAV-SC4

Plasmid

#236254

Purpose

AAV-SC4 capsid which targets peripheral nerves in mice

Depositor

Casey Maguire

Article

Abou Haidar et al Gene Ther. 2025 Jun 10. doi: 10.1038/s41

Inserts

rep/cap AAV9 VP1 with GATPSTQ peptide insert
rep

Use

AAV

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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EIF2AK4 gRNA (BRDN0001146469)

Plasmid

#75876

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK1 gRNA (BRDN0001147731)

Plasmid

#77388

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK1 (CDK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-SARS-CoV-2

Plasmid

#236423

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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