We narrowed to 879 results for: PFL
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Plasmid#133395PurposePiggybac vector for canonical Wnt signaling reporterDepositorInserttdTomato-NLS
ExpressionMammalianMutationN/APromoterSuperTOPFlashAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating vā¦Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TPD54
Plasmid#172450PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal FLAG tagDepositorAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF1C-A-RFP
Plasmid#172431PurposeStrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059ā1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNA500
Plasmid#149583Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF2C-A-RFP
Plasmid#172432PurposeStrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059ā1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC12)_Psyn-sgRNArodA
Plasmid#149661Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC12), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only