We narrowed to 851 results for: gfp cassette

1. pDiCre-mCherry

   Plasmid

   #164572

   Purpose

   Integration of a DiCre cassette in PbGFP parasite genome

   Depositor

   Olivier Silvie

   Article

   Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

   Inserts

   FKBP12-CRE59
   FRB-CRE60
   mCherry
   Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Bidirectional PbEF1alpha promoter (PBANKA_1133300…

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SOX17-NLS-tdTomato-EPG

   Plasmid

   #210466

   Purpose

   Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

   Plasmid

   #179402

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd(DW890/891AA)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Phd domain DW890/891AA

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. prCOrev

   Plasmid

   #153961

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. prCO

   Plasmid

   #153960

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-chr20-49345720-gRNA1

   Plasmid

   #232623

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiGuide-chr20-49345720-gRNA2

   Plasmid

   #232624

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiGuide-chr20-49345720-gRNA3

   Plasmid

   #232625

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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