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Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only
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pTrex-Luc-Phleo
Plasmid#48337PurposeTrasgenically expresses firefly luciferase in T. cruzi with phleomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCMV 16E6no* delPDZ
Plasmid#47678PurposePDZ-binding motif is deletedDepositorInsertHPV 16 E6no* (E6 HPV, Human)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7aa and last 6aa, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMV261-igr3K253E
Plasmid#225739PurposeTo express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in MycobacteriaDepositorInsertMyc-igr3K253E
UseTagsc-MycExpressionBacterialMutationigr3 K253EPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-EcYRS-EGFP*
Plasmid#191118PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafishDepositorInsertMutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV261-igr3WT
Plasmid#225738PurposeTo express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in MycobacteriaDepositorInsertMyc-igr3WT
UseTagsc-MycExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-Bxb1-Xis-mScarlet
Plasmid#195570PurposeExpresses Bxb1 excisionase fused with mScarlet for fluorescenceDepositorInsertBxb1 Excisionase Fused With mScarlet
UseSynthetic BiologyTagsmScarletExpressionMutationPromoterP7Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopTagsExpressionMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTet-Bxb1-CFP
Plasmid#195374PurposeExpresses Bxb1 integrase fused with CFP for fluorescenceDepositorInsertBxb1 Integrase Fused With deCFP
UseSynthetic BiologyTagsdeCFPExpressionMutationPromoterpTetAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-21
Plasmid#195058Purposesimplyfied amino acid sequence by alanine scanningDepositorInsertbovine pancreatic trypsin inhibitor
UseTagsHis tag and trpĪLE leader sequencesExpressionBacterialMutation21 amino acids are Alanine out of 58 amino acidsPromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MtrxRFP2
Plasmid#172323Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInserthuman thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
UseTagsHis-tagExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
UseTags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX2AHK
Plasmid#118826PurposeRetroviral expression of a mutant of Sox2 that improves efficiency of iPSC generationDepositorInserteSox2AHK (Sox2 Mouse)
UseRetroviralTagsExpressionMammalianMutationAHK: E46A, I53HPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPa-c6MYCn
Plasmid#69242PurposeTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-c6MYCn
Plasmid#69243PurposeTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only