Skip to main content
Addgene
Showing: 1641 - 1660 of 6991 results

  6. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid
    #234371
    Purpose
    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis Ubi10 and MTAP1 (synthetic)
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234371-plasmid-map-sequence-id-469425

    Enlarge

  8. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222853
    Purpose
    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222853-plasmid-map-sequence-id-445015

    Enlarge

  9. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222855
    Purpose
    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222855-plasmid-map-sequence-id-445010

    Enlarge

  10. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

    Enlarge

  12. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid
    #191333
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191333-plasmid-map-sequence-id-381754

    Enlarge

  13. pBetaActin-FKBP-Dync1h1motor

    Plasmid
    #191332
    Purpose
    Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Dync1h1motor (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191332-plasmid-map-sequence-id-381753

    Enlarge

Showing: 1641 - 1660 of 6991 results