We narrowed to 13,284 results for: sequence
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Plasmid#209868PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddGFP A
TagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
TagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHUE411
Plasmid#62203PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-ICUE3
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorInsertLyn-ICUE3 (RAPGEF3 Human)
TagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ173
Plasmid#99907PurposeExpress CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertpco-dCas9-VP64-T2A-MS2-VP64
UseCRISPRExpressionPlantAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-Flag-natT1R2
Plasmid#113944Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839Available SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only