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Plasmid#111504PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC588 - pAAV c-fos Nuc-iRFP
Plasmid#59132PurposeAn AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.DepositorInsertNuclear-localized Infrared Fluorescent Protein
UseAAVTags3xNLSExpressionMammalianMutationPromotermFosAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>{H2B-RFP}
Plasmid#201121PurposeRFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclearDepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationNoPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS2CP(V29I)-myc-T2A-tagRFP
Plasmid#140260PurposeExpresses MS2CP(V29I) in mammalian cellsDepositorInsertCoat protein of bacteriphage MS2
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR pUAS-dGFP pPGK-iRFP
Plasmid#176530PurposeLentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.DepositorInsertsdGFP
iRFP
UseLentiviralTagsExpressionMammalianMutationPromoterPGKp and UASpAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-miRFP670-3XNLS-WPRE
Plasmid#159952Purposenucleus markerDepositorInsertmiRFP670-3XNLS
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-LexGAD-3xP3-RFP
Plasmid#127558PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertLexGAD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4DBD-3xP3-RFP
Plasmid#127564PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4DBD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-QF2-3xP3-RFP
Plasmid#127559PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.DepositorInsertQF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP720-P2A-GFP
Plasmid#197165PurposeExpresses the protein of miRFP720-P2A-GFP in mammalian cellsDepositorInsertmiRFP720-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse c-MycV394D-IRES-RFP
Plasmid#73573PurposeMammalian retroviral expression of mouse Myc V394D mutantDepositorInsertcMyc (Myc Mouse)
UseRetroviralTagsIRES-RFPExpressionMammalianMutationV394DPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-GFP-iRFP670-Tdtomato
Plasmid#111813PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
iRFP670
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-iRFP670-GFP-Tdtomato
Plasmid#111816PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
GFP
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-iRFP
Plasmid#176028Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720DepositorInsertstetR
iRFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
BsaI-tagRFP-BsaI-pUC-kan
Plasmid#170771Purposetest plasmid for Golden Gate assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)
Plasmid#117856PurposeThis plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
V1664 pLPS-3'RFP-mito - Acceptor
Plasmid#11702DepositorTypeEmpty backboneUseCre/Lox; Acceptor vectorTagsRFP-mitoExpressionMammalianMutationPromoterAvailable sinceMay 26, 2006AvailabilityAcademic Institutions and Nonprofits only