We narrowed to 5,049 results for: golden gate
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Plasmid#253022PurposeEncodes the Suc2 signal peptide, Invertase, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable markerDepositorInsertSuc2
ExpressionYeastAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD274
Plasmid#253021PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable markerDepositorInsertmRuby2
ExpressionYeastAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD095
Plasmid#252985PurposeEncodes GFP11-M3 as a Type 4a part to be used for fluoresence complementation based detection in the yeast secretion/display (YSD2.0) MoClo toolkit Fluoresence ComplementationDepositorInsertGFP11-M3
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD862
Plasmid#253015PurposeEncodes Anti-mCherryLaM-4 Nanobody as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertAnti-mCherry_LaM-4 Nanobody
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD861
Plasmid#253014PurposeEncodes Anti-ALFA-tag Nanobody as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertAnti-ALFA-tag Nanobody
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD052
Plasmid#252965PurposeEncodes S. cerevisiae HA Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2DepositorInsertAga2
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD058
Plasmid#252971PurposeEncodes S. cerevisiae MYC Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2DepositorInsertAga2
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD069
Plasmid#252973PurposeEncodes Anti-GFP Nanobody (no tag) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertAnti-GFP Nanobody
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD047
Plasmid#252963PurposeEncodes S. cerevisiae Pir2 anchor-linker-MYC Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertPir
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD051
Plasmid#252964PurposeEncodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2DepositorInsertAga2
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD094
Plasmid#252984PurposeEncodes GFP11-M3-His6-tag as a Type 4a part to be used for fluoresence complementation based detection in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertGFP11-M3-His6-tag
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD045
Plasmid#252961PurposeEncodes S. cerevisiae Pir anchor-linker-HA Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertPir
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYSD046
Plasmid#252962PurposeEncodes S. cerevisiae Pir anchor-linker-2xFLAG Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)DepositorInsertPir
ExpressionBacterialAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pICH41331_NOS-PPT
Plasmid#232550PurposeLevel-0 module; Phosphinothricin gene regulated by Nos promoter and terminatorDepositorInsertPhosphinothricin gene regulated by Nos promoter and terminator
ExpressionPlantAvailable SinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔVSΔREP-RT-pDGB3alpha1
Plasmid#240727PurposeBCTV vector containing the GUS:NPTII repair template for HDR experiments. Lacks the BCTV virion sense ORFs and Rep has been mutated.DepositorInsertRT
ExpressionPlantMutation2 stop codons inserted in RepAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAV035_mCherry_P2A_fsEGFP_GGsite
Plasmid#219807PurposeFluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34DepositorInsertmCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
(155-bp-DIAL-YB_TATA)-mGL-BGH_pKG2846
Plasmid#246330Purpose155-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTL51_cHS4-CAG-s36GFPHaloTag-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4
Plasmid#223542PurposePUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cellsDepositorInserts36GFP-HaloTag, mCherry-3NLS, PAC
ExpressionMammalianPromoterCAGAvailable SinceJan. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
(263-bp-DIAL-YB_TATA)-mGL-BGH_pKG2847
Plasmid#246331Purpose263-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
LentiX_(203-bp-DIAL-YB_TATA)-mGL-BGH_pKG2945
Plasmid#246342Purpose203-bp DIAL Reporter Lentivirus expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseLentiviral and Synthetic BiologyAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-nCas9-PolI5M
Plasmid#249066PurposeExpresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertnCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-enCas9(D10A)-PolI5M
Plasmid#249060PurposeExpresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-dCas9-PolI5M
Plasmid#249065PurposeExpresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertdCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG1526
Plasmid#246357PurposeZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the EF1a promoterDepositorInsertVP16-ZF43-2A-TagBFP
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiX_((203-bp-DIAL-YB_TATA)-mGL-BGH)rev_DIV_pKG2948
Plasmid#246343Purpose203-bp DIAL Reporter Lentivirus (with divergent iRFP670) expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseLentiviral and Synthetic BiologyAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-VP16-ZF37-2A-mCherry-BGH_pKG1501
Plasmid#246355PurposeZFa Plasmid expressing VP16-ZF37-2A-mCherry from the EF1a promoterDepositorInsertVP16-ZF37-2A-mCherry
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-VP16-ZF37-2A-TagBFP-BGH_pKG3223
Plasmid#246356PurposeZFa Plasmid expressing VP16-ZF37-2A-TagBFP from the EF1a promoterDepositorInsertVP16-ZF37-2A-TagBFP
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(79-bp-DIAL-YB_TATA)-mGL-BGH_pKG1523
Plasmid#246329Purpose79-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(nested-DIAL-YB_TATA)-mGL-BGH_pKG2876
Plasmid#246332Purposenested DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre and VCre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-SV40)-mGL-BGH_pKG3687
Plasmid#246334Purpose203-bp DIAL Reporter Plasmid with SV40 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-minCMV2)-mGL-BGH_pKG3680
Plasmid#246335Purpose203-bp DIAL Reporter Plasmid with minCMV2 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-JB42CAT5)-mGL-BGH_pKG3688
Plasmid#246336Purpose203-bp DIAL Reporter Plasmid with JB42CAT5 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-MLP)-mGL-BGH_pKG3681
Plasmid#246337Purpose203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-CMV53)-mGL-BGH_pKG3682
Plasmid#246338Purpose203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-YB_TATA)-mGL-BGH_pKG1525
Plasmid#246326Purpose203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(27-bp-DIAL-YB_TATA)-mGL-BGH_pKG1524
Plasmid#246328Purpose27-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3
Plasmid#242154PurposeExpression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassetteDepositorInsertAraC R2 S170 LOV P1.1.3
ExpressionBacterialMutationAraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…Available SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFC482
Plasmid#246439PurposeThe plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signalDepositorInsertCasE
TagsNLSExpressionMammalianMutationSapI domesticatedPromoterEF-1aAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV824
Plasmid#246453PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV823
Plasmid#246452PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV746
Plasmid#246449PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV745
Plasmid#246448PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV785
Plasmid#246451PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1326
Plasmid#246058PurposeBacterial expression of RFP under control of the pJEX promoterDepositorInsertRed Fluorescent Protein
ExpressionBacterialPromoterpJEX promoterAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTB105
Plasmid#236778PurposeBackbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1739
Plasmid#246064PurposeBacterial expression of mCpol under control of the pTet promoterDepositorInsertMinimal CRISPR polymerase
ExpressionBacterialPromoterpTet promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1543
Plasmid#246062PurposeBacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoterDepositorInsertAnti CBASS 2
ExpressionBacterialMutationY8APromoterpJEX promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1866
Plasmid#246069PurposeBacterial expression of N4gp48 under control of the pJex promoterDepositorInsertAnti CBASS 4
ExpressionBacterialPromoterpJex promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1398
Plasmid#246059PurposeBacterial expression of T4 Acb1 under control of the pJex promoterDepositorInsertAnti CBASS 1
ExpressionBacterialPromoterpJEX promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1399
Plasmid#246060PurposeBacterial expression of T4 Acb2 under control of the pJex promoterDepositorInsertAnti CBASS 2
ExpressionBacterialPromoterpJEX promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only