Addgene

We narrowed to 6,446 results for: poly

Showing: 2521 - 2550 of 6446 results

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pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Use

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAB213

Plasmid

#101677

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTHSSd_38

Plasmid

#59961

Purpose

High core fragment expression (high resource allocator)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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hTRAF2T117ApLPCX-HA-Flag/P875

Plasmid

#20230

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

T117A (a349g in TRAF2 CDS)

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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hTRAF2K31RpLPCX-HA-Flag/P876

Plasmid

#20231

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K31R (a92g in the TRAF2 CDS sequence).

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-23P639

Plasmid

#51388

Depositor

Narry Kim

Article

Lee et al EMBO J. 2004 Oct 13;23(20):4051-60. Epub

Insert

miR-23a promoter region (MIR23A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pCNPpo6-H98A

Plasmid

#46087

Depositor

Raymond Monnat

Article

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Insert

PpoI H98A

Use

Tags

NLS

Expression

Mammalian

Mutation

catalytically inactive H98A variant

Promoter

CMV

Available since

June 21, 2014

Availability

Academic Institutions and Nonprofits only

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BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pQCXIP-Rx3(A6)-mEGFP

Plasmid

#35530

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A6)mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac FPSC

Plasmid

#1926

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

posterior sex combs (Psc Fly)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Use

Tags

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGMEH-P38-luxAB2

Plasmid

#27061

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Use

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT5

Plasmid

#27051

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
pTHSSd_23

Plasmid

#59954

Purpose

PTac T7 sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134288

Plasmid

#152098

Purpose

Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pQCXIP-Rx3(A8)-mEGFP

Plasmid

#35532

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3A8mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pTHSSd_44

Plasmid

#59966

Purpose

constitutive expression of T7 sigma fragment

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Pcon sigma

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT1

Plasmid

#27053

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-ssrAec

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCY 3090-02

Plasmid

#36231

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEpolylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-hphMX4

Expression

Mutation

Promoter

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_25

Plasmid

#59956

Purpose

PTac K1FR sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

K1FR sigma fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_26

Plasmid

#59957

Purpose

PTac CGG sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

CGG sigma fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134323

Plasmid

#152878

Purpose

Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTHSSd_29

Plasmid

#59959

Purpose

PTac alpha fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

alpha fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046219

Plasmid

#153926

Purpose

Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046319

Plasmid

#153866

Purpose

Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTHSSd_1

Plasmid

#59947

Purpose

Splitposon in KanR ColE1 backbone

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Splitposon w/ KanR, LacI & PTac

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGFH14A5

Plasmid

#39542

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 96-100 changed to alanine

Promoter

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only