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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50455

Purpose

Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

Depositor

Bryan Roth

Has Service

AAV8

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

human Synapsin 1

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

Plasmid

#129496

Purpose

Lentiviral expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

MND

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

Plasmid

#209410

Purpose

Expression of wild-type neonatal isoform of human SCN2A.

Depositor

Al George

Article

Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

Insert

SCN2A Variant 4, stabilized (SCN2A Human)

Use

Tags

Expression

Mammalian

Mutation

IVS intron inserted after bp 4551 of the SCN2A OR…

Promoter

CMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Use

Tags

Expression

Mammalian

Mutation

T27stop

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1365 LV EF1a-CD28 IRES-EGFP

Plasmid

#201921

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD28 (CD28 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Expression

Mutation

Delta gag/pol

Promoter

HIV LTR

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

Plasmid

#122966

Purpose

Expresses 3XFlag-tagged human CEP164 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

CEP164 (CEP164 Human)

Use

Lentiviral

Tags

3XFlag

Expression

Mutation

Promoter

EF1 alpha

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

Plasmid

#208696

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

Plasmid

#203907

Purpose

Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4LOV(SK22)-VP64-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HNRNPA1

Plasmid

#200727

Purpose

Lentiviral expression of Flag-Tagged human HNRNPA1

Depositor

Claudio Alarcón

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pTIGHT

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT K78E-IRES-Puro

Plasmid

#182591

Purpose

expresses TERT K78E in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78E

Promoter

EF-1a

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203658

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

Plasmid

#193298

Purpose

episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV CAMKII PSAM4 GlyR IRES EGFP

Plasmid

#119744

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CamKII

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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