162,300 results

1. gRNA Cloning Vector Bbs I ver. 2

   Plasmid

   #85586

   Purpose

   An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL2299

   Plasmid

   #186332

   Purpose

   This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

   Depositor

   Kan Wang

   Article

   Kang et al Front Plant Sci. 2022 May 4;13:860971. d

   Insert

   virulence genes from Agrobacterium tumefaciens pTiBo542
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-LC3-YFP

   Plasmid

   #99571

   Purpose

   Lentiviral expression of YFP-tagged LC3

   Depositor

   Edward Campbell

   Article

   Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubiquitin WT

   Plasmid

   #12647

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubiquitin - wild type (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. iLID-mCl3-MAP7N

   Plasmid

   #190167

   Purpose

   Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   iLID-mClover3-MAP7N
   

   Use

   Tags

   iLID-mClover3

   Expression

   Mammalian

   Mutation

   aa 1-309 from human MAP7 delta175-213

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. TPC1-G-GECO1.2

   Plasmid

   #207143

   Purpose

   Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPCN1 (TPCN1 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sniper2L

   Plasmid

   #193856

   Purpose

   Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-HuC(elavl3)-CaMPARI2

   Plasmid

   #137185

   Purpose

   pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
   

   Use

   Tol2 plasmid for zebrafish

   Tags

   FLAG, HA, myc

   Expression

   Mutation

   Promoter

   HuC(elavl3)

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1983

    Plasmid

    #143562

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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