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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKL2299

Plasmid

#186332

Purpose

This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

Depositor

Kan Wang

Publication

Kang et al Front Plant Sci. 2022 May 4;13:860971. d

Insert

virulence genes from Agrobacterium tumefaciens pTiBo542

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-TO-MYOD1-shOct4

Plasmid

#182309

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

MYOD1-shOct4 (MYOD1 Human)
puro-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

shOct4

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-N-RAM-d2tTA-TRE-mKate2

Plasmid

#140275

Purpose

N-RAM, the Npas4-dependent reporter

Depositor

Yingxi Lin

Publication

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Has service

AAV1

Insert

mKate2

Use

AAV

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Publication

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Scaffold gRNA

Expression

Mammalian

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEHlyA5

Plasmid

#168298

Purpose

Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

Depositor

Luis Ángel Fernández

Publication

Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

Insert

N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA

Tags

E-tag, HA-tag, His-tag, Prescission site, and VHH…

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-SFPQ

Plasmid

#166959

Purpose

Expresses HA-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Publication

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

Plasmid

#154983

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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