We narrowed to 875 results for: seph

1. TMEM138-mCherry

   Plasmid

   #41630

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

   Plasmid

   #171102

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Otx2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG AKT3 E17K;K177M

   Plasmid

   #73049

   Purpose

   Human AKT3 E17K;K177M

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K;K177M (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E17K;K177M

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA TMEM 138-flag

   Plasmid

   #41628

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-Mito-Car-GECO

   Plasmid

   #100765

   Purpose

   Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   Lenti-Mito-Car-GECO
   

   Use

   Lentiviral

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

   Plasmid

   #217676

   Purpose

   AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.

   Depositor

   Joseph Raimondo

   Insert

   GtACR2-P2A-Voltron2ST
   

   Use

   AAV

   Tags

   Soma targeting sequence (Kv2.1) on Voltron2 gene …

   Expression

   Mammalian

   Mutation

   Promoter

   EF‐1α

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYeDP60-hATP13A2-WT

   Plasmid

   #171377

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Use

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   Promoter

   URA3

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

   Plasmid

   #171101

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Olig2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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