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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAN1935

Plasmid

#127203

Purpose

Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1

Use

Synthetic Biology

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Promoter

Ef1a

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAN1232

Plasmid

#127164

Purpose

Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)

Depositor

Hana El-Samad

Article

Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1

Use

Synthetic Biology

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAN1572

Plasmid

#127202

Purpose

Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1

Use

Synthetic Biology

Available Since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Tip alpha (28-192)

Plasmid

#23158

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-27

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pDMG1a

Plasmid

#33158

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
p7138 pHAGE-P-CMVt-N-HA-GAW-PHKB

Plasmid

#100158

Purpose

Expresses PHKB

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

PHKB (PHKB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFM112

Plasmid

#40158

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1C:FLAG (par-1 Nematode)

Tags

GST and modified Flag

Expression

Bacterial

Mutation

Contains aa 966-1192

Promoter

tac

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pet29(b+) AIcrVIA2

Plasmid

#231126

Purpose

Plasmid for bacterial expression and purification of AIcrVIA2

Depositor

Gavin Knott

Article

Taveneau et al Nat Chem Biol. 2026 Jan 26. doi: 10.1038

Insert

AicrVIA2

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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P2A-mCherry-pA-ORF-1 without ATG

Plasmid

#241158

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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L4872 VP64-intN in PolyTX-mNeonGreen

Plasmid

#244191

Purpose

Constitutive expression of VP64-intN split synTF component

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

Split synTF component VP64-intN

Use

Synthetic Biology

Tags

3xFLAG - nuclear localization sequence

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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L4873 intC-ZF9 in PolyTX-mTagBFP2

Plasmid

#244192

Purpose

Constitutive expression of intC-ZF9 split synTF component

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

Split synTF component intC-ZF9

Use

Synthetic Biology

Tags

nulcear localization sequence - HA

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCA2)

Plasmid

#235069

Purpose

Expresses Tad-CBE(CCA2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCA2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT1)

Plasmid

#235076

Purpose

Expresses Tad-CBE(GCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT2)

Plasmid

#235077

Purpose

Expresses Tad-CBE(GCT2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG1)

Plasmid

#235079

Purpose

Expresses Tad-CBE(GCG1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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